金银三角形纳米环结构的制备及其传感研究

发布时间：2018-02-27 09:36

本文关键词： 局域表面等离子体共振三角形银纳米粒子三角纳米环结构双氧水的测定葡萄糖的测定　出处：《苏州大学》2015年硕士论文　论文类型：学位论文

【摘要】：贵金属纳米材料如金、银等有较为独特的局域表面等离子体共振(LSPR)特性,它们大部分可以应用于催化、生物传感以及医药等领域。与球形纳米粒子相比,三角形银纳米粒子的LSPR吸收峰对周围介质折射率变化极其敏感。糖尿病是一个世界性的公共卫生问题,已被认为是导致死亡的全球性疾病之一。糖尿病的诊断和控制需要密切地监测血液中葡萄糖的浓度。传统的光度分析法是用葡萄糖氧化酶或辣根过氧化酶等,且采用有机显色剂来测定。本论文合成了金银三角纳米环结构,并应用于比色法测定葡萄糖,金银三角纳米环首先可以代替葡萄糖氧化酶催化氧化葡萄糖,接着利用生成的过氧化氢刻蚀环中的银原子使其结构发生变化,从而引起金银三角环LSPR吸收峰的变化。由此可便捷地用该方法检测葡萄糖,避免使用任何酶及显色剂。论文第二章是采用晶种生长的方法制备三角形银纳米粒子。首先是用循环伏安法在ITO玻璃表面直接电沉积银纳米种子,然后将制备好的银纳米晶种放在生长液中生长,形成三角形结构。利用紫外-可见吸收分光光度法、扫面电镜等方法对其表征。三角形结构的银纳米粒子紫外吸收峰在650nm左右。同时讨论了生长液中各试剂浓度、生长时间及温度对形成三角形结构的影响。论文第三章是在溶液中合成金银三角纳米环结构,并将其应用于LSPR生物传感。根据文献报道采用双氧水在溶液中合成三角形银纳米粒子,在上述合成的溶液中加入还原剂抗坏血酸等生成金银三角纳米环,并对其进行表征;同时考察了不同试剂加入量和反应时间对反应的影响。将金银三角纳米环结构应用于测定双氧水及葡萄糖浓度,得出三角形环纳米结构测定双氧水和葡萄糖时分别在一定的浓度范围内具有良好的线性。测定葡萄糖时其线性范围500ppb~5ppb,检测下限(LCD)达4uM,相关系数为0.99884。以人体血液为实际样品测定,回收率达93.4%-102%。由此证实了金银三角形环纳米结构传感器具有无试剂且方便的优点,因此制备这类传感器具有十分重要的意义。
[Abstract]:Noble metal nanomaterials such as gold and silver have unique local surface plasmon resonance (LSPRs) properties, which can be used in catalysis, biosensor, medicine and other fields. The LSPR absorption peak of triangular silver nanoparticles is extremely sensitive to the refractive index changes in the surrounding media. Diabetes is a worldwide public health problem. Diabetes diagnosis and control require close monitoring of blood glucose concentrations. Traditional photometric analysis uses glucose oxidase or horseradish peroxidase, etc. In this paper, the structure of gold and silver triangulated rings was synthesized and applied to the determination of glucose by colorimetric method. The gold and silver triangular rings can be used to catalyze the oxidation of glucose instead of glucose oxidase. Then the structure of the silver atom in the ring was changed by hydrogen peroxide etching, which caused the change of the LSPR absorption peak of the gold and silver triangulation ring. This method can be used to detect glucose conveniently. In chapter 2, triangular silver nanoparticles were prepared by seed growth. First, silver nanoparticles were electrodeposited on the surface of ITO glass by cyclic voltammetry. Then the prepared silver nanocrystalline seeds were grown in the growth solution to form a triangular structure. UV-Vis absorption spectrophotometry was used. The UV absorption peak of triangular silver nanoparticles was about 650 nm. The concentration of reagents in the growth solution was also discussed. The influence of growth time and temperature on the formation of triangular structure. According to the literature, triangular silver nanoparticles were synthesized by hydrogen peroxide in the solution, and the gold and silver triangulated rings were formed by adding ascorbic acid into the above synthetic solution, and the gold and silver triangular rings were characterized. At the same time, the effects of different reagents and reaction time on the reaction were investigated. The gold and silver triangular nanospheres were used to determine the concentration of hydrogen peroxide and glucose. The results showed that the linear range of hydrogen peroxide and glucose was in a certain concentration range. The linear range of glucose was 500 ppb ~ 5 ppb, the detection limit was 4 渭 M, and the correlation coefficient was 0.99884.The results showed that the linear range was 500 ppb ~ (5) ppb, and the correlation coefficient was 0.9988 4. The recovery rate is 93.4-102. It is proved that the gold and silver triangular ring nanosensors have the advantages of no reagent and convenience, so the preparation of this kind of sensors is of great significance.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O614.12;TB383.1

【共引文献】