长径比可控银棒@聚丁二炔核壳结构的制备及与牛血清蛋白复合物的研究

发布时间：2018-03-10 03:38

本文选题：核壳复合纳米材料　切入点：银棒　出处：《延边大学》2017年硕士论文　论文类型：学位论文

【摘要】：核壳复合纳米材料主要综合了无机材料和有机材料的优良特性,并不是简单的将两种或两种以上的组分进行叠加,除了保证每种单独组分的特点,而且会得到新的特征性能。银纳米棒具有独特光学性质,具体表现为横向和纵向的表面等离子共振(SPR)吸收峰。聚丁二炔(Polydiacetylene,PDA)是一类独特的π-共轭聚合物。PDA单体在受到紫外光照下,会变成无荧光的蓝色相,当受到加热等外部刺激时会变成有荧光信号的红色相。正因为这种特性广泛应用于比色/生物传感器。但由于PDA的荧光量子产率较低,阻碍了其进一步的应用。金属荧光增强(Metal Enhanced Fluorescence,MEF)可以解决这个问题。影响MEF现象的因素主要有两点,一是金属粒子与荧光团之间的距离在5到15 nm,二是荧光团的发射频率与金属纳米粒子的SPR的匹配程度。本文以晶种法合成银纳米棒,并且通过控制银纳米晶种的大小,调整加入晶种的量,改变生长液中各组分之间的浓度,生长出长径比在2左右可调控长度的银纳米棒。PDA的激发光谱与加入30 μL的晶种所生长银纳米棒的SPR峰匹配程度最高,PDA荧光效果增强。通过加入NH4SCN蚀刻银棒,得到空心的PDA壳结构,与银棒@聚丁二炔核壳结构相比,证实银纳米棒的存在,PDA的荧光会得到增强。利用静电吸附法,合成银棒@聚丁二炔核壳结构。此时银棒与PDA之间的距离较短,在3 nm左右。水热法合成银棒@聚丁二炔核壳结构,使银纳米棒与PDA之间的距离变大,到8 nm左右。也考察与牛血清蛋白(BSA)的结合,随着银棒@聚丁二炔浓度的增加,发生荧光猝灭现象。并得到稳定的BSA-Ag@PDA复合物,预测这种长径比可控的银棒@聚丁二炔核壳结构在生物成像等领域具有潜在的应用价值。
[Abstract]:Core-shell composite nanomaterials mainly integrate the excellent properties of inorganic and organic materials, and are not simply superimposed on two or more components, except to ensure the characteristics of each individual component. The silver nanorods have unique optical properties, such as transverse and longitudinal surface plasmon resonance (SPR) absorption peaks. Poly (butylene diacetylene) PDAs are a kind of unique 蟺 -conjugated polymers. PDA monomers are exposed to ultraviolet light. It turns into a blue phase without fluorescence, and becomes a red phase with a fluorescent signal when it is stimulated by external stimuli such as heating. This property is widely used in colorimetric / biosensors, but because of the low fluorescence quantum yield of PDA, Metal fluorescence enhanced metal Enhanced fluorescence can solve this problem. There are two main factors that influence the MEF phenomenon. One is that the distance between metal particles and fluorescent groups is between 5 and 15 nm, and the other is the match between the emission frequency of fluorescent clusters and the SPR of metal nanoparticles. In this paper, silver nanorods are synthesized by the method of crystal seeding, and the size of silver nanocrystalline seeds is controlled. Adjust the amount of seed added, change the concentration of each component in the growth solution, The excitation spectra of silver nanorods grown with aspect ratio of about 2 can be adjusted to match the SPR peak of silver nanorods grown with 30 渭 L crystal seeds. The fluorescence effect of PDAs was enhanced by adding NH4SCN etched silver bars. The hollow PDA shell structure was obtained. Compared with the silver rod / polybutylene core shell structure, the fluorescence of the silver nanorods was confirmed to be enhanced. The distance between silver rod and PDA is about 3 nm. Hydrothermal synthesis of silver rod @ polybutylene core shell structure increases the distance between silver nanorods and PDA. At about 8 nm, the binding of BSA with bovine serum protein (BSA) was also investigated. With the increase of the concentration of silver bar @ polybutylene, the fluorescence quenching phenomenon occurred, and the stable BSA-Ag@PDA complex was obtained. It is predicted that this kind of silver rod / polybutylene core-shell structure with controllable aspect ratio has potential application value in biological imaging and other fields.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB333

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