金纳米粒子的表面修饰及组装和解组装性质的研究

发布时间：2018-03-13 07:18

  本文选题：球形金纳米粒子　切入点：金纳米棒　出处：《东北师范大学》2015年硕士论文　论文类型：学位论文

【摘要】：利用DNA分子对金纳米粒子进行表面修饰和通过DNA的配对实现纳米粒子的自组装的研究已经相当广泛。考虑到DNA的配对是通过碱基的配对完成的,因此单独采用带有碱基的有机小分子配体修饰金纳米粒子,并且利用核酸碱基间的互补配对(特异性识别-氢键作用)实现金纳米粒子在溶液中进行组装,并通过控制温和条件实现解组装,从而赋予金纳米粒子以开关功能。具体内容如下:1、本课题设计合成了带有不同长度烷基链和硫醇官能团的腺嘌呤(A)和胸腺嘧啶(T)配体。通过柠檬酸钠还原氯金酸的方法制备了球形金纳米粒子,并且利用自主合成的A-C12-SH配体和T-C12-SH配体对球形金纳米粒子进行表面修饰。实验表明,配体的浓度影响被修饰的球形金纳米粒子的聚集,A-C12-SH配体和T-C12-SH配体的浓度高会引起金纳米粒子的聚集。p H值对配体修饰球形金纳米粒子的研究表明,在p H6的酸性条件下,A-C12-SH配体修饰金纳米球后,会发生粒子的聚集;在p H4的强酸性条件下,T-C12-SH配体修饰的金纳米球会发生聚集。经过A-C12-SH配体和T-C12-SH配体修饰后的金纳米球之间可以发生自组装,并且通过调节溶液的酸碱性,可以控制金纳米球的组装与解组装。2、通过晶种生长法,控制生长溶液中Ag NO3的含量制备出了纵向吸收峰为780nm的金纳米棒,并且利用自主合成的A-C12-SH配体和T-C12-SH配体对金纳米棒进行表面修饰。通过紫外分析得出在p H≤6的酸性条件下,经过A-C12-SH配体修饰的GNR会发生横向聚集;在p H≥10的强碱性条件下,T-C12-SH配体修饰的GNR发生横向聚集。将带有T-C12-SH配体的金纳米棒和带有A-C12-SH配体的金纳米球进行组装,当修饰的金纳米球比例大于修饰的金纳米棒,组装会形成团聚,紫外吸收不再有纵向吸收峰;当修饰的金纳米棒的比例大于修饰的金纳米球的比例,组装后的紫外吸收峰仍然有纵向吸收峰;并且酸性和碱性条件都会加大组装程度。
[Abstract]:The surface modification of gold nanoparticles using DNA molecules and the self-assembly of gold nanoparticles by DNA pairing have been extensively studied. Considering that the pairing of DNA is accomplished by base pairing, Therefore, gold nanoparticles were modified by organic small molecular ligands with bases alone, and the gold nanoparticles were assembled in solution by complementary pairing between nucleic acid bases (specific recognization-hydrogen bond interaction). And by controlling the mild conditions to realize the disassembly, The specific contents are as follows: 1. In this paper, we designed and synthesized adenine (A) and thymine (Thymine) ligands with different alkyl chains and mercaptan functional groups. Chloruronic acid was reduced by sodium citrate. The spherical gold nanoparticles were prepared by the method of. The spherical gold nanoparticles were modified by self-synthesized A-C12-SH ligands and T-C12-SH ligands. The effect of concentration of ligand on the aggregation of gold nanoparticles, such as the concentration of A-C12-SH ligands and T-C12-SH ligands, will lead to the aggregation of gold nanoparticles. After modification of gold nanospheres with A-C12-SH ligands under acidic conditions of pH _ 6, the aggregation of gold nanoparticles will occur. The gold nanospheres modified by T-C12-SH ligands can aggregate under the strong acidity of pH4. The gold nanospheres modified by A-C12-SH ligands and T-C12-SH ligands can be self-assembled and can be modified by adjusting the acidity and basicity of the solution. The gold nanorods with a longitudinal absorption peak of 780nm were prepared by controlling the content of Ag NO3 in the growth solution by controlling the assembly and unassembly of gold nanospheres. The gold nanorods were modified by self-synthesized A-C12-SH ligands and T-C12-SH ligands. UV analysis showed that the GNR modified by A-C12-SH ligands would aggregate laterally under acidic pH 鈮，

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