金纳米簇的制备及其应用于金属离子的检测研究

发布时间：2018-03-17 09:19

本文选题：聚集诱导发光效应　切入点：金纳米簇　出处：《郑州大学》2016年硕士论文　论文类型：学位论文

【摘要】：贵金属纳米簇具有独特的光学性质,具有毒性低、水溶性高、稳定性强以及生物相容性好等优点,这使其在检测分析和生物成像等方面有广阔的应用前景。因此,近年来,这种新型纳米材料,受到了研究者们广泛的关注。在本论文中,我们制备了水溶性的金纳米簇,并用其作为纳米荧光探针,实现了对金属离子快速、简单的检测分析。研究内容主要包括以下三个方面:1、分别用牛血清白蛋白(Bovine Serum Albumin,简称BSA)、人血清白蛋白(Human Serum Albumin,简称HSA)、半胱氨酸(Cysteine,简称Cys)以及谷胱甘肽(Glutataione,简称GSH)作为弱还原剂及保护剂合成金纳米簇,并对合成的金纳米簇性质进行了研究,包括粒径、光稳定性、耐酸碱性、量子产率以及细胞毒性等。2、基于聚集诱导发光(AIE)性质,我们合成的Cys-AuNCs作为一种非标记的光学探针来检测Ca~(2+)。当Cys-AuNCs和Ca~(2+)孵育后,因Cys-Ca~(2+)的相互作用,Cys-AuNCs就迅速聚集,形成荧光点亮的聚集体。通过荧光的变化可以灵敏、特异地检测Ca~(2+)的浓度,这是一种成本低、设计简单以及响应快速的非标记探针。3、GSH-AuNCs被作一种Pb~(2+)可视化检测的探针。当GSH-AuNCs与Pb~(2+)孵育时,因GSH会与Pb~(2+)作用,GSH-AuNCs会在1 min内迅速地形成聚集体,从而引起荧光的显著增强。增强的荧光强度与Pb~(2+)浓度存在线性关系。在12种二价金属离子中,该探针对Pb~(2+)具有很高的选择性。同时可以用便携式紫外灯实现对污染物Pb~(2+)的现场测试。
[Abstract]:Noble metal nanoclusters have unique optical properties, low toxicity, high water solubility, high stability and good biocompatibility. In this paper, we have prepared water-soluble gold nanoclusters and used them as nano-fluorescent probes to achieve a rapid response to metal ions. The main contents of this study are as follows: 1: 1. Bovine Serum albumin (BSA), Human Serum albumin (HSA), cysteine cysteine (Cys) and glutathione Glutataione (Glutataione) are respectively used as BSA. Synthesis of Gold Nanoclusters with weak reducing agents and Protective agents, The properties of the synthesized gold nanoclusters, including particle size, photostability, acid and alkalinity resistance, quantum yield and cytotoxicity, were studied based on agglomeration induced luminescence (AIEs) properties. Our synthesized Cys-AuNCs is used as an unlabeled optical probe for the detection of Ca~(2. When Cys-AuNCs and Ca~(2 are incubated, Cys-AuNCs gather rapidly due to the interaction of Cys-Ca~(2) and form fluorescent brightening aggregates. A low cost, simple, and fast response non-labeled probe. GSH-AuNCs is used as a Pb~(2) visual detection probe. When GSH-AuNCs is incubated with Pb~(2, Because GSH acts with Pb~(2, GSH-AuNCs rapidly form aggregates within 1 min, which leads to a significant enhancement of fluorescence. There is a linear relationship between the enhanced fluorescence intensity and the concentration of Pb~(2. In 12 divalent metal ions, there is a linear relationship between the enhanced fluorescence intensity and the concentration of GSH-AuNCs. The probe is highly selective for Pb~(2) and can be used for field testing of contaminant Pb~(2 by portable UV lamp.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O614.123;TB383.1

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