碳纳米材料和银纳米簇的合成和应用

发布时间：2018-04-13 19:38

本文选题：碳点 + 金黄色葡萄球菌　；参考：《湖南科技大学》2017年硕士论文

【摘要】：作为新型碳纳米材料,碳点(CDs)具有优良性能,被广泛地应用于多个领域。CDs的粒径普遍在3~5 nm之间,表面含有各类功能化基团,且可通过改变功能化集团来调整CDs的性质。相对于传统的半导体量子点来说,CDs具备更好的光学性能、生物相容性和环境友好性,能够应用于生物成像、纳米传感、光催化、光电子器件等范围。本文基于细菌自身具有较均匀的特征,以其为碳源,利用水热法或微波辅助制备出性能优良的荧光CDs,并用于离子检测等各个方面:(1)本文以金色葡萄糖球菌为碳源,结合水热法合成技术成功制备了均匀性较好的CDs,经提纯后得到具备荧光特性的CDs溶液。获得的CDs在350 nm的激发下,于440nm处有一荧光发射峰。根据内滤效应,在酸性条件下,CDs可以用来检测盐酸小檗碱。当CDs溶液与盐酸小檗碱溶液反应后,CDs溶液的荧光会发生猝灭,猝灭值与盐酸小檗碱的溶液浓度呈线性关系(线性方程为(35)I=3.79×106c+16.02,r=0.9972),线性范围为1.0×10-7-7.5×10-5 mol/L,检测限为7.03×10-8(3σ/k)。基于此线性关系,能够检测盐酸黄连素片样品中的盐酸小檗碱含量。(2)本文以酵母细菌为主碳源,乙二胺为辅助碳源,微波快速合成CDs溶液,获得有荧光特性的CDs溶液;获得的CDs在390 nm的激发下,于460 nm处有一荧光发射峰。该CDs能够用于溶液pH的检测。CDs溶液与不同pH的溶液反应后,CDs溶液的荧光强度值与溶液的pH之间呈线性关系(IF=259.90-12.61 pH,r=0.9936),检测的线性范围为线性范围为1.81~11.82。(3)本文以羧甲基葡聚糖为定稳剂和还原剂,紫外灯照射[Ag(NH3)2]OH溶液获得一种新的溶于水溶性荧光银纳米簇;并对其大小和形貌进行了TEM、HRTEM、荧光和紫外光谱表征。基于银纳米簇与半胱氨酸之间的相互作用,导致银纳米簇荧光猝灭,猝灭值与半胱氨酸的溶液浓度呈线性关系(线性方程(35)I=4.25×109c+36.84,r=0.9967),线性检测范围为5.0×10-10 1.0×10-7 mol/L,检测限为3.16×10-10(3?/k)。基于此线性关系,建立了一种可用于半胱氨酸检测的荧光传感器。
[Abstract]:As a new carbon nanomaterials, carbon dots (CDs) have been widely used in many fields. The diameters of CDs are generally between 3nm and 5nm. The surface contains various functional groups, and the properties of CDs can be adjusted by changing the functionalized groups.Compared with traditional semiconductor quantum dots, CDs have better optical properties, biocompatibility and environmental friendliness. They can be used in biological imaging, nanosensor, photocatalysis, optoelectronic devices and so on.In this paper, based on the homogeneous characteristics of bacteria, the fluorescent CDS with excellent properties were prepared by hydrothermal method or microwave-assisted method using them as carbon source, and they were used in ion detection and other aspects. In this paper, Glucococcus aureus was used as carbon source, and Glucococcus aureus was used as carbon source.CDs with good homogeneity was successfully prepared by hydrothermal synthesis. After purification, the CDs solution with fluorescence characteristics was obtained.Under the excitation of 350nm, the obtained CDs has a fluorescence emission peak at 440nm.According to the internal filtration effect, CDs can be used to detect berberine hydrochloride under acidic conditions.After the reaction of CDs solution with berberine hydrochloride solution, the fluorescence of CDs solution will be quenched. The quenching value is linear with the concentration of berberine hydrochloride solution (linear equation is 3.79 脳 10 ~ (-6) C ~ (16.02C) ~ (0.9972)), the linear range is 1.0 脳 10 ~ (-7) -7.5 脳 10 ~ (-5) mol 路L ~ (-1), the detection limit is 7.03 脳 10 ~ (-8) ~ (-3) 蟽路K ~ (-1).Based on this linear relationship, berberine hydrochloride content in berberine hydrochloride tablets can be determined. In this paper, yeast bacteria as the main carbon source, ethylenediamine as the auxiliary carbon source, microwave rapid synthesis of CDs solution, to obtain fluorescence characteristics of CDs solution;Under the excitation of 390nm, the obtained CDs has a fluorescence emission peak at 460nm.The CDs can be used to detect pH of solution. After reaction between CDs solution and solution with different pH, there is a linear relationship between fluorescence intensity value of CDs solution and pH value of solution.Sugar is stabilizer and reductant,A novel water-soluble fluorescent silver nanocluster was obtained by ultraviolet lamp irradiation with [Ag(NH3)2] OH solution, and its size and morphology were characterized by TEM HRTEM, fluorescence and UV spectra.Based on the interaction between silver nanoclusters and cysteine, the fluorescence quenching of silver nanoclusters was induced. The quenching value was linearly related to the concentration of cysteine solution (linear equation = 3.35 脳 10 ~ (-9) c ~ 36.84C ~ (0.9967)). The linear detection range was 5.0 脳 10 ~ (-10) ~ (-10) ~ (-1) mol 路L ~ (-1), and the detection limit was 3.16 脳 10 ~ (-10) ~ (3) C ~ (-1) / kg ~ (-1).Based on the linear relationship, a fluorescence sensor for detection of cysteine was established.
【学位授予单位】：湖南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O657.3

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