磁介导功能型纳米粒子在铁支架表面定向捕获CD34阳性细胞

发布时间：2018-04-20 21:06

  本文选题：内皮祖捕获 + 干细胞　； 参考：《中国科学:技术科学》2017年05期

【摘要】：构建内皮祖细胞捕获支架是目前心血管疾病治疗的一个研究热点.利用磁性纳米粒子捕获内皮祖细胞也越来越受到关注,但是单纯的磁性纳米粒子和磁场源地作用相对微弱,本研究引入铁支架作为另一磁响应源,共同作用来提高纳米粒子表面捕获率.首先利用CD34抗体构建了一种定向捕获CD34阳性细胞的功能型磁性纳米粒子,再利用外加磁场将其归巢到铁支架表面.FTIR和TEM结果表明CD34抗体已成功接枝到双羧基PEG包裹的Fe_3O_4纳米粒子上,体外细胞评价证实该纳米粒子不具有细胞毒性.体内动物实验结果表明该Fe_3O_4-PEG@CD34纳米粒子能够特异性识别捕获CD34阳性细胞,并且在外加磁场的作用下能够将其快速归巢到铁支架表面,这为实现支架表面快速内皮化提供了可能.
[Abstract]:The construction of endothelial progenitor cell capture stent is a hot topic in the treatment of cardiovascular disease. The capture of endothelial progenitor cells by magnetic nanoparticles has attracted more and more attention, but the effect of magnetic nanoparticles and magnetic field source is relatively weak. In this study, iron scaffolds were introduced as another magnetic response source. The surface trapping rate of nanoparticles can be improved by interaction. First, a functional magnetic nanoparticles was constructed by using CD34 antibody to capture CD34 positive cells. The results of homing to the surface of iron scaffold. FTIR and TEM showed that CD34 antibody had been successfully grafted onto the Fe_3O_4 nanoparticles coated with dicarboxylic PEG. The cell evaluation in vitro confirmed that the nanoparticles were not cytotoxic. The results of animal experiments in vivo show that the Fe_3O_4-PEG@CD34 nanoparticles can specifically recognize and capture CD34 positive cells, and can quickly homing them to the surface of the iron scaffold under the action of external magnetic field, which provides the possibility for the rapid endothelialization of the scaffold surface.
【作者单位】： 西南交通大学材料先进技术教育部重点实验室;西南交通大学生命科学与工程学院;
【基金】：国家自然科学基金(批准号:81271701)资助项目
【分类号】：R54;TB383.1
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本文编号：1779434