表面活性剂功能化金纳米棒在生物识别中的应用及其自组装行为研究

发布时间：2018-04-28 10:34

本文选题：金纳米棒 + 表面活性剂　；参考：《北京化工大学》2016年硕士论文

【摘要】：金纳米棒作为一种特殊结构的金纳米颗粒,拥有独特的局部表面等离子体共振特性。最常用的金纳米棒合成方法为阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)作为模板剂和稳定剂的种子诱导生长法。然而CTAB功能化的金纳米棒具有一定的生物毒性,且稳定性和选择性差,很难应用于生物体系研究或实际临床检测。因此需要进一步对其进行表面功能化。本文主要利用非离子表面活性剂和阴离子表面活性剂分别对金纳米棒进行表面修饰,研究其对金纳米棒的稳定性及分散性等性质的影响,拓展其在生物小分子分析检测中的应用,并进行金纳米棒自组装行为研究。主要研究内容如下：(1)研究了非离子含氟表面活性剂(FSN)对金纳米棒在生物小分子检测中的重要作用。研究发现FSN功能化的金纳米棒不仅具有很好的稳定性、抗盐性、抗酸碱性和生物相容性,同时FSN能够在金纳米棒表面产生空间位阻效应,屏蔽其他含硫化合物的干扰,通过金-硫键作用力实现对生物硫化氢的高选择性、高灵敏度的检测,最低检出限为0.2μM。同时利用这一比色传感体系评估一种硫化氢合成酶胱硫醚-p-合成酶的活性,并将该方法应用于人和小鼠血清样品中生物硫化氢含量的实际检测。(2)研究了不同阴离子表面活性剂与阳离子表面活性剂CTAB功能化金纳米棒的之间的相互作用以及对金纳米棒分散性的影响。发现由于阴阳离子表面活性剂之间的疏水作用和静电作用,金纳米棒会随着阴离子表面活性剂添加浓度的改变产生不同程度的聚集和分散效果,同时混合时间的增长也会引起金纳米棒的聚集。通过控制阴离子表面活性剂浓度和反应混合时间,可以利用十二烷基磺酸钠(SDS)得到一种新型、分散性好、表面带负电的阴离子表面活性剂功能化金纳米棒。此外,合成阴离子表面活性剂TPE-SDS,利用其特殊结构和静电作用,改变了半胱氨酸诱导金纳米棒的自组装行为。不但提升了自组装速率,同时形成了新型的金纳米棒网状自组装结构。
[Abstract]:As a kind of special structure gold nanoparticles, gold nanorods have unique local surface plasmon resonance (SPR) characteristics. The most commonly used method for the synthesis of gold nanorods is the seed induced growth of cationic surfactant cetyltrimethylammonium bromide (CTAB) as template and stabilizer. However, the CTAB functionalized gold nanorods have some biological toxicity, and the stability and selectivity are poor, it is difficult to be used in biological system research or practical clinical detection. Therefore, it needs further surface functionalization. In this paper, Nonionic surfactants and anionic surfactants were used to modify the surface of gold nanorods, and the effects of Nonionic surfactants and anionic surfactants on the stability and dispersion of gold nanorods were studied. Its application in the analysis and detection of biological small molecules was expanded, and the self-assembly behavior of gold nanorods was studied. The main research contents are as follows: (1) the important role of Nonionic fluorine-containing surfactant (FSNs) in the detection of small biomolecules by gold nanorods is studied. It was found that FSN functionalized gold nanorods not only have good stability, salt resistance, acid and alkalinity resistance and biocompatibility, but also that FSN can produce steric resistance on the surface of gold nanorods and shield other sulfur compounds from interference. The high selectivity and high sensitivity of hydrogen sulfide can be achieved by gold-sulfur bond force. The lowest detection limit is 0.2 渭 M. At the same time, the colorimetric sensing system was used to evaluate the activity of a hydrogen sulfide synthase cystathithione-p- synthase. The method was applied to the determination of hydrogen sulfide in human and mouse serum samples. The interaction between different anionic surfactants and cationic surfactant CTAB functionalized gold nanorods was studied. The effect on the dispersion of gold nanorods. It is found that due to the hydrophobic and electrostatic interaction between anionic and anionic surfactants, gold nanorods have different aggregation and dispersion effects with the change of the concentration of anionic surfactants. At the same time, the increase of mixing time will also lead to the aggregation of gold nanorods. By controlling the concentration of anionic surfactants and reaction mixing time, a new type of anionic surfactant functionalized gold nanorods with good dispersity and negative charge on the surface can be obtained by using sodium dodecyl sulfonate (SDS). In addition, the synthesis of anionic surfactant TPE-SDS changed the self-assembly behavior of gold nanorods induced by cysteine by using its special structure and electrostatic action. It not only improves the self-assembly rate, but also forms a new self-assembly structure of gold nanorods.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TB383.1;O614.123

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