基于离子液体表面活性剂微乳液体系淀粉纳米微球的制备及性质研究

发布时间：2018-05-10 23:34

本文选题：表面活性剂 + 离子液体微乳液　；参考：《华南理工大学》2015年硕士论文

【摘要】：淀粉作为一种天然高分子材料,早期没有被作为药物载体原材料,主要是因为其水溶性差,不容易成球。以淀粉或其衍生物为原料制备淀粉微球,能够使其作为药物载体在生物体中缓慢降解释放药物,延长药物达到体内的作用时间,提高生物利用度。如果将淀粉微球制备成纳米级,由于淀粉纳米微球具有巨大的比表面积,颗粒具有较高的胶体稳定性和吸附性能,能够作为药物载体材料在药物传输系统中得到广泛应用。本文从离子液体微乳液的制备和性质研究、基于离子液体微乳液体系淀粉纳米微球的制备和表征及淀粉纳米微球的载药和释药性能等方面,对淀粉纳米微球的制备及性质进行了系统研究,以期为淀粉纳米微球的制备提供一条环保、高效的新方法,拓展淀粉纳米微球在生物医药领域的应用。分别制备了C16mim Br/正丁醇/环己烷/水和C16mim Br/正丁醇/环己烷/[Omim]Ac两种不同类型离子液体微乳液,通过稀释实验、动态光闪射技术、拟三元相图、电导率等考察了微乳液的性质,证明了油包离子液体(W/O)和离子液体包水(IL/O)两种微乳液体系的形成。分别以W/O和IL/O微乳液体系为反应体系制备了淀粉纳米微球。采用扫描电镜(SEM)、动态光散射(DLS)技术、X-射线衍射(XRD)和红外光谱(FTIR)对淀粉纳米微球的结构及性质进行了研究。SEM结果表明,淀粉纳米微球为球形颗粒,颗粒尺寸较小;DLS结果显示,淀粉纳米微球的粒径较小,粒径分布范围较窄;XRD图谱表明,淀粉纳米微球的结晶结构消失,呈无定型结构;FTIR数据证明了淀粉分子间交联键的形成。以淀粉纳米微球为载体,亚甲基蓝和盐酸米托蒽醌为模型药物,分别考察了时间、温度和药物浓度对淀粉纳米微球载药性能的影响。结果表明,随着载药时间的增加,淀粉纳米微球的载药量和包封率先增大后减小;随着温度的升高,淀粉纳米微球的载药量和包封率也同样先增大后减小;随着药物浓度的增加,淀粉纳米微球的载药量逐渐增大,而包封率先增加后减小。载药淀粉纳米微球的释药过程主要包括两个阶段:快速释放阶段和缓慢释放阶段,淀粉纳米微球表现出一定的缓释性能。
[Abstract]:Starch, as a kind of natural polymer material, was not used as the raw material of drug carrier in the early stage, mainly because of its poor water solubility and difficult to be pelletized. Starch microspheres prepared from starch or its derivatives can be used as drug carriers to slowly degrade and release drugs in organism, prolong the time of drug reaching in vivo, and improve the bioavailability. If starch microspheres are prepared into nanometer scale, because of their huge specific surface area, high colloidal stability and adsorption performance, they can be widely used as drug carrier materials in drug transport system. In this paper, the preparation and properties of ionic liquid microemulsion, the preparation and characterization of starch nanospheres in ionic liquid microemulsion system, and the drug loading and drug release properties of starch nanoparticles were studied. The preparation and properties of starch nanospheres were systematically studied in order to provide a new method for the preparation of starch nanospheres and to expand the application of starch nanospheres in the field of biomedicine. Two different types of ionic liquid microemulsions, C16mim Br/ n-butanol / cyclohexane / water and C16mim Br/ n-butanol / cyclohexane / [Omim] ac, were prepared respectively. The properties of microemulsions were investigated by dilution experiment, dynamic flicker technique, pseudo-ternary phase diagram, conductivity, etc. The formation of two microemulsion systems, W / O and IL / O, has been proved. Starch nanoparticles were prepared from W / O and IL/O microemulsion systems. The structure and properties of starch nanospheres were studied by scanning electron microscope (SEM), dynamic light scattering (DLS) technique and FTIR (FTIR). The results of SEM showed that the starch nanoparticles were spherical and the particle size was smaller than that of DLS. The results of XRD showed that the crystalline structure of starch nanospheres disappeared, and the FTIR data showed that the formation of cross-linking bonds between starch molecules was proved by FTIR data. The effects of time, temperature and drug concentration on the drug delivery properties of starch nanospheres were investigated by using methylene blue and mitoxantrone hydrochloride as model drugs. The results showed that the drug loading and encapsulation rate of starch nanoparticles increased first and then decreased with the increase of drug loading time, and the drug loading and encapsulation efficiency of starch nanoparticles increased first and then decreased with the increase of temperature. With the increase of drug concentration, the drug loading of starch nanoparticles increased gradually, while the encapsulation increased first and then decreased. The drug release process of drug loaded starch nanoparticles mainly includes two stages: rapid release phase and slow release phase. Starch nanoparticles show a certain sustained release performance.
【学位授予单位】：华南理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TS236.9;TB383.1

【参考文献】