基于简单结构及双信号放大的量子点基电化学发光免疫传感器的建立与应用

发布时间：2018-05-15 03:01

本文选题：电化学发光免疫分析 + 免疫传感器　；参考：《苏州大学》2015年硕士论文

【摘要】：电化学发光免疫分析是电化学发光(E C L)和免疫分析相结合的产物。作为一种新型的分析检测手段,电化学发光免疫方法因具有高灵敏度、高特异性的特点,已经被广泛地应用于各种领域。本文制备了水溶性的量子点作为发光体以及石墨烯胶体金等新型的纳米材料,在此基础上建立了一种简单结构的电化学发光免疫传感器以及一种双信号放大的电化学发光免疫传感器,分别对食品中的受体激动剂沙丁胺醇、苯乙醇胺A以及重金属汞离子进行了快速、灵敏的检测,对拓展基于量子点的电化学发光免疫方法的应用有着重要的意义。本论文主要包括五个方面:1.介绍论文的研究背景及意义。阐述电化学发光免疫分析的基本理论及类型,着重归纳可以提高电化学发光免疫分析灵敏度的发光标记物及固定化载体。2.制备了巯基乙酸(T G A)为稳定剂的水溶性C d S e量子点、胶体金以及连有正电荷P D D A的还原型氧化石墨烯。借助紫外可见吸收(U V-v i s)光谱、高分辨率投射电镜(T E M)、扫描电镜(S E M)等表征手段对制备的材料进行了表征。3.利用L-半胱胺和戊二醛,通过简单的A u-S键、-N H2与-C H O的特征官能团之间的反应,以相对较短的链连接包被抗原和抗体,层层修饰,得到电化学发光免疫传感器,并用于检测沙丁胺醇。本实验最低检出限为0.0 0 5 6 n g/m L,具有良好的稳定性及特异性。4.利用本实验室制备的对二价汞离子具有高特异性的6-巯基烟酸的甲基汞包被抗原及单克隆抗体,通过简单结构的电化学发光免疫传感器对汞离子进行了检测。最低检出限为0.0 0 6 2 n g/m L,明显低于传统的测定方法。对实际样品的检测进一步证明该方法的可行性,同时为重金属的检测提供了新的思路。5.通过静电作用创造性地制备了P-G R-Q D s-A u N P s-A b复合物探针,该探针增加了发光体量子点的量,提高了检测苯乙醇胺A的灵敏度。借助紫外可见吸收(U V-v i s)光谱、高分辨率投射电镜(T E M)、扫描电镜(S E M)等表征手段对该复合物探针进行了表征。
[Abstract]:Electrochemiluminescence immunoassay (ECL) is a combination of ECL and immunoassay. As a new analytical and detection method, electrochemiluminescence immunoassay has been widely used in various fields because of its high sensitivity and specificity. In this paper, water-soluble quantum dots (QDs) were prepared as luminescent materials and new nano-materials such as graphene colloidal gold. A simple electrochemical luminescent immunosensor and a double signal amplified electrochemical luminescent immunosensor were developed for the treatment of salbutamol, a receptor agonist in food, respectively. The rapid and sensitive detection of phenylethanolamine A and heavy metal mercury ions is of great significance for the development of electrochemiluminescence immunoassay based on quantum dots. This thesis mainly includes five aspects: 1. This paper introduces the research background and significance. The basic theory and types of electrochemiluminescence immunoassay (ECLIA) were described, and the luminescent markers and immobilized carrier. Water soluble C d S e quantum dots, colloidal gold and reduced graphene oxide with positively charged P D D A were prepared with thioglycolic acid (TGA) as stabilizer. The prepared materials were characterized by UV-vis absorption U V-v I S), high resolution projection electron microscopy (TEM), scanning electron microscopy (SEM) and so on. By using L- cysteamine and glutaraldehyde, electrochemiluminescence immunosensors were obtained by the reaction between simple characteristic functional groups of A-S bond, N-H _ 2 and -C H _ O, which were coated with antigens and antibodies with relatively short chains. It was used for the detection of salbutamol. The lowest detection limit of this experiment is 0.056 n / g / mL, which has good stability and specificity. 4. Mercury ion was detected by a simple electrochemical luminescent immunosensor using methylmercury coated antigen and monoclonal antibody of 6-mercaptonicotinic acid prepared in our laboratory. The lowest detection limit was 0.062 ng / mL, which was significantly lower than that of the traditional method. The feasibility of the method is further proved by the detection of actual samples, and a new way of thinking is provided for the detection of heavy metals. The P-G R-Q D s-A u N P s-A b complex probe was creatively prepared by electrostatic action. The probe increased the quantity of luminescent quantum dots and improved the sensitivity of detecting phenylethanolamine A. The composite probe was characterized by UV-vis absorption U V-v I S), high resolution projection electron microscopy (TEM), scanning electron microscopy (SEM) and so on.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TP212

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