云母晶面上准外延生长的胶原蛋白纳米线阵列的性质研究

发布时间：2018-06-03 17:40

本文选题：胶原蛋白 + 外延生长　；参考：《分析化学》2017年04期

【摘要】：胶原蛋白纤维与同样具有周期性结构表面的羟磷灰石等矿物质晶体能够通过多种作用在介观尺度上相互识别,从而有效地进行规范骨骼、牙齿等器官生长的生物矿化。在本研究中,反相利用生物矿化原理,开发出一种与热壁外延生长(Hot wall epitaxy)技术效果相似的生物大分子纳米线阵列的简单制备技术,成功地将5~10μg/m L的天然I型鼠尾胶原蛋白单体溶液在云母晶体的(001)晶面上培育成取向单一且长程有序的胶原蛋白纳米线阵列。原子力显微镜实验结果表明,纳米线阵列随胶原蛋白单体浓度增大而变得致密,但是单条纳米线的宽度与高度较为稳定,分别约为60.0和1.5 nm。有纳米线覆盖的云母晶面亲水性好,晶面接触角由25.8°降为9.5°。基于电子背面散射衍射和透射电子显微镜的分析表征结果,纳米线的取向应沿云母[ˉ~110]方向,更详实地验证了胶原蛋白纳米线的准外延生长机理。
[Abstract]:Collagen fibers and mineral crystals such as hydroxyapatite, which also have periodic structures, can be recognized on mesoscopic scales through various interactions, thus effectively biomineralizing the growth of bone, teeth and other organs. In this study, using the principle of biomineralization, a simple fabrication technique of biomolecular nanowire arrays was developed, which is similar to that of hot-wall epitaxial growth wall arrays. The natural rat tail collagen monomer solution of 5 渭 g / mL was successfully grown on the crystal plane of mica crystal to form a single and long term ordered collagen nanowire array. Atomic force microscopy (AFM) results showed that the nanowires became compact with the increase of collagen monomer concentration, but the width and height of the single nanowires were stable, about 60.0 and 1.5 nm, respectively. The mica crystal surface covered by nanowires has good hydrophilicity, and the contact angle of the crystal plane is reduced from 25.8 掳to 9.5 掳. Based on the analysis and characterization of electron backscattering diffraction and transmission electron microscope, the orientation of nanowires should be along the direction of mica. The quasi-epitaxial growth mechanism of collagen nanowires is verified in detail.
【作者单位】：南京理工大学电子工程与光电技术学院;
【基金】：国家自然科学基金(No.51303082) 中央高校基本科研业务费专项资金(No.30915011316) 江苏省“高层次创新创业人才引进计划”项目资助~~
【分类号】：TB383.1

【相似文献】