基于纳米金辅助信号生成顺序堆积的毛细管电泳免疫分析研究

发布时间：2018-06-17 08:38

本文选题：短裸甲藻毒素B + 毛细管电泳　；参考：《化学学报》2017年04期

【摘要】：短裸甲藻毒素(BTX)是具有极强毒性的生物毒素,能够通过食物链传递引起人类中毒.由于该毒素没有光学和电化学信号,检测十分困难.本工作利用纳米金作为载体,将辣根过氧化物酶(HRP)和毒素抗体同时固定到纳米金表面,通过HRP催化H_2O_2氧化邻氨基酚(OAP)产生的电化学信号检测样品中的毒素,增大纳米金表面HRP和抗体的物质的量比使电化学信号得到极大增强.免疫反应样品电动进样引入分离毛细管中,在毛细管入口端进行顺序堆积在线富集,使检测灵敏度进一步提高.该方法通过纳米金辅助信号生成和顺序堆积在线富集技术实现了对扇贝样品中BTX-B的快速灵敏检测,线性范围为0.1~120 ng/mL,检出限为26 ng/L,检出限比常规酶联免疫分析(ELISA)法低365倍.
[Abstract]:BTX is a highly toxic biotoxin which can be transmitted through the food chain to cause human poisoning. Because the toxin does not have optical and electrochemical signals, detection is very difficult. In this work, using gold nanoparticles as carrier, horseradish peroxidase (HRP) and toxin antibody were immobilized on the surface of gold nanoparticles at the same time. The electrochemical signals produced by H _ 2O _ 2 O _ 2O _ 2 oxidation of o-aminophenol OAP) were used to detect the toxins in the samples. The electrochemical signal was greatly enhanced by increasing the ratio of HRP and antibody on the surface of nanocrystalline gold. The immunoreactive sample was introduced into the separation capillary and sequentially stacked at the inlet of the capillary. The detection sensitivity was further improved. The rapid and sensitive detection of BTX-B in scallop samples was achieved by means of nanocrystalline gold assisted signal generation and sequential stacking on-line enrichment technique. The linear range of BTX-B in scallop was 0.1 ~ 120ng / mL, and the detection limit was 26 ng / L, which was 365 times lower than that by conventional enzyme linked immunosorbent assay (ELISA).
【作者单位】：青岛科技大学化学与分子工程学院肿瘤标志物传感分析教育部重点实验室;
【基金】：国家自然科学基金(No.21105051)资助~~
【分类号】：TB383.1;O658.9

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