单一金纳米电极的制备及应用

发布时间：2018-06-22 07:49

本文选题：金纳米盘电极 + 单一纳米线电极　；参考：《安徽师范大学》2015年硕士论文

【摘要】：近年来,随着对纳米电极研究的深入,纳米电极日益凸显它的优势,灵敏度高,响应快,检测限低,更小的RC常数以及更快的传质速率。因为其在电极尺寸上的优势,纳米电极可应用于研究电子传递动力学,检测单个纳米离子,作为探针用于SECM,生物活体及单细胞分析。众所周知,金的表面由于具有更好的反应活性使得金电极有更多的应用,比如说表面活性自组装修饰,电催化,生物分析等等。在本文中,已经制备半径小于5nm的金纳米盘电极以及单一金纳米线电极,并且讨论了其电化学行为,也研究了其在电化学生物传感器方面的应用。论文主要包括以下四个章节:第一章:纳米电极的概述,双金属核-壳结构,DNA简介,电化学DNA生物传感器的研究进展第二章:通过激光拉制的方法首次制备了半径小于5nm的金纳米盘电极,电化学表征,场发射透射电子显微镜(HR-TEM)和X-射线能谱分析法(EDX)对纳米电极进行了表征,电极表面修饰铁蛋白的直接电化学研究了纳米电极在电化学传感方面的应用。第三章:由金纳米盘电极,通过氟化氢刻蚀技术制备成单一金纳米线电极(Au-SPNEs)。再通过欠电位沉积(UPD)铜氧化置换技术制成铂壳金核的金纳米线电极(Au@Pt-SPNEs),PtCl42-置换UPD的铜层。场发射透射电子显微镜(HR-TEM)、X-射线能量射线谱分析法(EDX)和电化学的方法对纳米线电极进行了表征。实验表明我们制备的Au@Pt-SPNEs电极对甲醇、氧气和双氧水都有很好的催化效果。第四章:制备了基于金纳米线电极(Au-SPNEs)的DNA生物传感器。以亚甲基蓝为电活性指示剂,采用方波伏安法进行检测,该DNA生物传感器很好的融合了“无试剂”污染和电化学的高灵敏响应的优点。这种新型的DNA传感器将潜在的应用于活体细胞中目标分析物的实时检测。
[Abstract]:In recent years, with the in-depth study of nano-electrode, nano-electrode increasingly highlights its advantages, such as high sensitivity, fast response, low detection limit, smaller RC constant and faster mass transfer rate. Because of its advantage in electrode size, nano-electrode can be used to study electron transfer kinetics, detect single nanoscale ion, and be used as probe for SECM, biological in vivo and single cell analysis. It is well known that the surface of gold has more applications due to its better reactive activity, such as surface active self-assembly modification, electrocatalysis, biological analysis and so on. In this paper, gold nanodisk electrode with radius less than 5nm and single gold nanowire electrode have been prepared. The electrochemical behavior of gold nanowire electrode and its application in electrochemical biosensor are also discussed. The thesis mainly includes four chapters as follows: chapter 1: Overview of nano-electrode, introduction of bimetallic core-shell structure DNA, Research Progress of Electrochemical DNA biosensor Chapter 2: gold nano-disk electrode with radius less than 5nm was prepared by laser pull method and characterized by electrochemistry. Field emission transmission electron microscopy (HR-TEM) and X-ray energy dispersive spectroscopy (edX) were used to characterize the nano-electrode. The application of nano-electrode in electrochemical sensing was studied by direct electrochemistry of ferritin modified on the electrode surface. Chapter 3: a single gold nanowire electrode (Au-SPNEs) was prepared by hydrogen fluoride etching. The au @ Pt-SPNEs / PtCl _ 42-replacement copper layer of au shell gold core was prepared by UPD copper oxidation replacement technique. The nanowire electrode was characterized by field emission transmission electron microscopy (HR-TEM) X-ray energy ray spectroscopy (edX) and electrochemical method. The experimental results show that the Autel-Pt-SPNEs electrode has good catalytic effect on methanol, oxygen and hydrogen peroxide. Chapter 4: a DNA biosensor based on Au-SPNEs was fabricated. Using methylene blue as the indicator of electrical activity and using square wave voltammetry, the DNA biosensor combines the advantages of "no reagent" pollution and high sensitivity of electrochemistry. This new type of DNA sensor will potentially be used for real-time detection of target analytes in living cells.
【学位授予单位】：安徽师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O646.54

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