表面连接与核-壳型键合FITC荧光纳米硅球的制备及性能比较

发布时间：2018-06-26 18:37

本文选题：异硫氰酸荧光素(FITC) + 荧光纳米硅球　；参考：《河北大学学报(自然科学版)》2017年03期

【摘要】：以异硫氰酸荧光素(FITC)为荧光分子,二氧化硅为生物相容性载体制备荧光纳米硅球.为增强硅球的稳定性,引入γ-氨丙基三乙氧基硅烷(APTES)做桥梁分子,由于FITC、SiO_2与APTES连接顺序的非单一性,分别制备了表面连接型和核-壳型键合FITC的荧光硅球,并对其形貌和光漂白性进行了比较.实验结果表明,核-壳型和表面连接型荧光硅球形貌相似粒径接近,但表面连接型光漂白性严重.进而,对核-壳型中典型的包硅方法——St9ber法和反相微乳液法进行比较,结果表明,反相微乳液法制备的核-壳型键合FITC的荧光硅球荧光信号更强、粒径更小且分散性良好,同时在7h内荧光染料泄漏程度小于8%,30min内荧光强度仅降低2.2%,具备低染料泄露、光稳定性好等优点.
[Abstract]:Fluorescent nanocrystals were prepared using fluorescein isothiocyanate (FITC) as fluorescent molecule and silica as biocompatibility carrier. In order to enhance the stability of silica spheres, 纬 -aminopropyltriethoxysilane (APTES) was introduced as a bridge molecule. Due to the non-singularity of the bonding sequence between FITCX SiO-2 and APTES, the fluorescent silica spheres bonded with FITC were prepared, respectively. The morphology and photobleaching properties were compared. The experimental results show that the morphologies of core-shell and surface-connected fluorescent silicon spheres are similar to each other, but the surface connected photolysis is very serious. Furthermore, the typical core-shell silicon inclusion method, St9ber method and inverse microemulsion method, are compared. The results show that the fluorescence signal of the core-shell bonded FITC is stronger, the particle size is smaller and the dispersion is good. At the same time, the leakage degree of fluorescent dye is less than 8% and the fluorescence intensity decreases only 2.2% within 30 min within 7 h, which has the advantages of low dye leakage and good light stability.
【作者单位】：河北大学化学与环境科学学院;
【基金】：河北省自然科学基金资助项目(B2016201213)
【分类号】：O613.72;TB383.1

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