酸性刺激响应智能纳米容器的制备及其可控释放研究

发布时间：2018-06-27 19:06

  本文选题：纳米容器 + 介孔二氧化硅纳米微球(MSNs)　； 参考：《南京理工大学》2016年硕士论文

【摘要】：纳米容器通常是指具有纳米尺寸并能够存储、释放目标物质的复合材料。由于介孔二氧化硅纳米微球(MSNs)具有包裹量大、表面易改性及生物无毒性等优点,已作为理想的纳米容器应用在生物医药领域中。特别是具有刺激响应性能的介孔二氧化硅纳米容器,通过外界刺激,纳米容器能够作出刺激响应,实现对客体分子的装载与释放。常见的外界刺激有pH、温度和氧化还原反应等,其中以pH作为外界刺激的研究最多。为此,本文以构建酸性刺激响应智能纳米容器药物载体为目标,开展了酸性刺激响应介孔二氧化硅纳米容器的制备及其药物分子的酸性可控释放研究,具体的工作如下。以S tober方法制得的介孔二氧化硅纳米微球(MSNs)作为纳米容器,吸附药物分子罗丹明B,首次使用新物质2-(2-溴-乙氧基)-2-(2-叠氮基-乙氧基)丙烷将氨基-p-环糊精连接到纳米容器的介孔处封堵罗丹明B,形成一种新型具有靶向抗癌作用的酸性智能纳米容器。采用扫描透射电子显微镜(SEM/TEM), X射线衍射(XRD)和比表面积分析(BET)等手段表征MSNs形貌和结构；使用固体核磁共振(SS NMR)、傅里叶红外光谱(FTIR)和热重分析(TGA)等手段验证了MSNs表面改性过程。利用荧光分光光度计(RF-5201)实时监测该智能纳米容器在不同酸性pH值下释放的动力学曲线；通过紫外分光光度计(UV-Vis)测得该智能纳米容器的载药量为15.23 mg/g。实验结果表明,利用末尾封端技术制得的以缩酮链作为刺激响应单元的智能纳米容器实现了在中性条件下“零释放”,酸性条件下大量释放(仿抗癌靶标溶酶体和内涵体条件下pH=4-5适量缓慢释放)的效果,此项研究在肿瘤细胞靶向方面具有很好的应用前景。同时又由于其具有在中性条件下“零释放”、酸性条件下大量释放的效果,使得该智能纳米容器也能在纳米催化、防腐涂层自修复等方面获得更好的应用。
[Abstract]:Nanovessels are usually composite materials that are nano-sized and can store and release the target material. Mesoporous silica nanospheres (MSNs) have been widely used in biomedical applications due to their advantages of large encapsulation, easy surface modification and biological nontoxicity. Especially for mesoporous silica nanovessels with stimulative response, the nanovessels can respond to the stimuli by external stimulation and realize the loading and releasing of guest molecules. The common external stimuli are pH, temperature and redox reaction, among which pH is the most widely studied. Therefore, the preparation of acidic stimulus-responsive mesoporous silica nanovessels and the controlled acidic release of drug molecules were studied in order to construct an intelligent drug carrier for acidic stimuli response. The main work is as follows. Mesoporous silica nanospheres (MSNs) prepared by S tober method were used as nano-containers. Rhodamine B, an adsorbed drug molecule, was used for the first time to bind amino-pcyclodextrin to the mesoporous site of the nano-container to block Rhodamine B with the new substance 2- (2-bromo-ethoxy) -2- (2-azido-ethoxy) propane. Action of the acid smart nano container. The morphology and structure of MSNs were characterized by scanning transmission electron microscopy (SEM / TEM), X-ray diffraction (XRD) and specific surface area analysis (BET), and the surface modification process of MSNs was verified by solid nuclear magnetic resonance (SS NMR), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). Fluorescence spectrophotometer (RF-5201) was used to monitor the release kinetics curve of the smart nano-vessel under different acidic pH, and the drug load of the smart nano-vessel was 15.23 mg / g by UV-Vis. The experimental results show that the intelligent nanovessel with Ketal chain as the stimulus response unit has achieved zero release under neutral conditions. The effect of a large amount of release (lysosome mimicking anticancer target and slow release of pH 4 to 5) under acidic conditions has a good prospect in the field of tumor cell targeting. At the same time, due to its effect of "zero release" in neutral condition and large amount of release in acidic condition, the intelligent nano-vessel can also be better applied in nano-catalysis and self-repair of anticorrosive coating.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TQ460.1;TB383.1

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