导电聚合物纳米复合材料的制备及其传感性能的研究

发布时间：2018-07-06 11:38

本文选题：生物传感器 + 肿瘤标记物　；参考：《青岛科技大学》2017年硕士论文

【摘要】：电化学传感器由于其潜在的高灵敏性、易于微型化、简便快捷、花费低等优点而受到人们广泛的关注,被广泛应用于生物医药、环境分析、生化分析等方面。但是,由于修饰材料对于电极的钝化作用以及蛋白质等生物大分子的非特异性吸附,使得生物传感器在实际样品检测方面的应用仍是一项巨大的挑战。为了解决上述两个问题,本研究工作成功制备了两种具有高灵敏性的电化学传感器。(1)首先,通过原位电聚合的方法成功制备了聚三,四-乙烯二氧噻吩(PEDOT)/聚并苯(PAS)纳米复合材料;得到的PEDOT/PAS纳米复合材料具有较大的比表面积和较多的活性位点,对于亚硝酸盐的催化氧化呈现较高的催化活性;在最优试验条件下,对于亚硝酸钠的检测范围为0.3μM到6.6 mM,最低检测限约为98 nM(S/N=3)。(2)随后,在基于亲水性材料聚多巴胺(pDA)、单宁酸(TA)、四臂聚乙二醇(PEG)基础上构建的抗蛋白非特异性吸附界面用于乳腺癌易感基因(BRCA1)的检测,线性范围为0.1 fM到10 pM,最低检测限约为0.05 fM(S/N=3)。而且,这两种传感器均可用于复杂环境中对于目标物的定量检测,并具有良好的稳定性和重现性。
[Abstract]:Electrochemical sensors are widely used in biomedicine, environmental analysis, biochemical analysis and so on because of their potential high sensitivity, easy miniaturization, simplicity and low cost. However, due to the passivation of modified materials to the electrode and the non-specific adsorption of biomolecules such as proteins, the application of biosensors in the field of sample detection is still a huge challenge. In order to solve the above two problems, two kinds of electrochemical sensors with high sensitivity have been successfully prepared in this work. (1) first of all, three kinds of electrochemical sensors have been successfully prepared by in situ electropolymerization. Tetraethylenedioxythiophene (PEDOT) / polyazobenzene (pas) nanocomposites have larger specific surface area and more active sites and exhibit higher catalytic activity for nitrite oxidation. Under the optimum test conditions, the detection range of sodium nitrite is from 0.3 渭 M to 6.6 mm, and the minimum detection limit is about 98 nm (S / N 3). (2). The nonspecific adsorption interface based on hydrophilic materials such as polydopamine (PDA), tannic acid (TA) and four arm polyethylene glycol (PEG) was used to detect breast cancer susceptibility gene (BRCA1). The linear range was from 0.1 FM to 10 pm, and the minimum detection limit was about 0.05 FM (S / N 3). Moreover, these two kinds of sensors can be used for quantitative detection of target in complex environments, and have good stability and reproducibility.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB33;O657.1

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