ICP-MS法测定添食金属纳米颗粒后家蚕组织中的金属含量

发布时间：2018-07-10 20:15

本文选题：家蚕 + 电感耦合等离子体质谱(ICP-MS)　；参考：《质谱学报》2017年02期

【摘要】：为研究添食金属或金属氧化物纳米颗粒后家蚕体内金属元素含量的变化规律,建立了微波消解结合电感耦合等离子体质谱(ICP-MS)测定家蚕血液、中肠和丝腺中Cu和Ti元素含量的方法。实验中分别给5龄3天家蚕喂食浓度为500.00mg/L纳米Cu和纳米TiO2悬浮液;72h后,剪破家蚕腹足收集家蚕血淋巴,并解剖收集中肠和丝腺。实验发现,Cu和Ti元素的标准曲线呈良好的线性关系,相关系数分别为0.999 95和0.999 89,两种元素的加标回收率均在96.6%~105.4%之间,相对标准偏差RSD小于3.7%。结果表明,对于纳米TiO2实验组,家蚕血液和丝腺中的Ti元素含量与空白组相比变化不大,而中肠的Ti元素含量明显增加为(181.46±9.58)μg/g,约为空白组含量(63.39±6.44)μg/g的3倍(p0.05);在纳米Cu组中,家蚕血液中的Cu元素含量有轻微的变化,而中肠和丝腺中的含量增加较多,分别为(82.73±1.72)μg/g和(3.88±0.17)μg/g,约为空白组含量(11.68±0.46)μg/g和(1.77±0.26)μg/g的7和2倍(p0.05)。该方法可准确地检测喂食纳米颗粒后,家蚕组织中的金属元素含量及其变化,可为进一步研究家蚕的纳米生物效应提供有力支持。
[Abstract]:In order to study the changes of metal element content in silkworm after feeding metal or metal oxide nanoparticles, microwave digestion combined with inductively coupled plasma mass spectrometry (ICP-MS) was established for the determination of silkworm blood. Methods for the content of Cu and Ti in midgut and silk gland. In the experiment, the silkworm was fed with 500.00mg / L nano-Cu and nano-TiO2 suspension for 72 hours, and the hemolymph of silkworm was collected by cutting the abdominal foot of silkworm, and dissecting the midgut and silk gland. It was found that the standard curves of Cu and Ti were linear, the correlation coefficients were 0.999 95 and 0.999 89, respectively. The recoveries of the two elements were between 96. 6% and 105. 4%, and the relative standard deviation (RSD) was less than 3. 7%. The results showed that the content of Ti in blood and silk gland of silkworm showed little change compared with the control group, but the content of Ti in midgut was significantly increased to (181.46 卤9.58) 渭 g / g, which was about 3 times of that in the blank group (63.39 卤6.44) 渭 g / g (p0.05). The contents of Cu in the blood of silkworm were slightly changed, but the contents in midgut and silk gland were (82.73 卤1.72) 渭 g / g and (3.88 卤0.17) 渭 g / g, respectively, which were 7 and 2 times of those in the blank group (11.68 卤0.46) 渭 g / g and (1.77 卤0.26) 渭 g / g (p0.05) respectively. This method can accurately detect the contents and changes of metal elements in silkworm tissues after feeding nanoparticles, which can provide strong support for further study on the biological effects of silkworm nanoparticles.
【作者单位】：江苏科技大学环境与化学工程学院;中国农业科学院蚕业研究所农业部蚕桑产品及食用昆虫质量安全风险评估实验室;
【基金】：江苏特聘教授科研基金(苏教师[2012]27)资助
【分类号】：Q5-33;TB383.1

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