比较叶酸和聚乳酸修饰Pluronic共聚物纳米粒子的细胞相容性

发布时间：2018-07-12 10:29

  本文选题：纳米粒 + 叶酸　； 参考：《中国组织工程研究》2017年14期

【摘要】：背景:研究发现,纳米材料与细胞接触时可诱导细胞产生氧化应激反应、过敏反应,进而产生细胞毒性和基因毒性,因此,对纳米毒理学的研究越来越受到广泛的关注。目的:比较Pluronic(P85,F127和F87)三嵌段共聚物经叶酸(folic acid,FA)和聚乳酸[poly(lactic acid),PLA]修饰前后的纳米结合物的细胞相容性。方法:对Pluronic三嵌段共聚物(P85,F127和F87)进行修饰,在其两端接上聚乳酸和叶酸,分别合成了多种两亲性嵌段共聚物(PLA-P85-PLA,FA-P85-PLA,PLA-F127-PLA,FA-F127-PLA,PLA-F87-PLA和FA-F87-PLA)纳米粒子。选取HepG-2细胞为研究对象,从细胞形态、细胞代谢活性、细胞膜效应来评价纳米粒子的细胞毒性。结果与结论:(1)未经叶酸和聚乳酸修饰的P85,F127和F87纳米粒子(5,10,20,50,100 mg/L)作用Hep G-2细胞24 h后,与对照组相比,细胞相对增长率差异无显著性意义(P0.05),且在低质量浓度范围内(5,10,20,50 mg/L)有促进细胞增殖的作用;当质量浓度增加到400 mg/L时,P85和F87纳米粒子对细胞增殖有明显的抑制作用;(2)经叶酸和聚乳酸修饰的P85,F127和F87纳米粒子(5,10,20,50,100,200,400 mg/L)作用Hep G-2细胞后,与对照组相比,差异无显著性意义(P0.05);(3)结果表明,用叶酸和聚乳酸修饰Pluronic(P85,F127和F87)三嵌段共聚物,可提高其细胞相容性,合成的PLA-Pluronic-PLA纳米粒子和FA-Pluronic-PLA纳米粒子可用作药物载体。
[Abstract]:Background: it has been found that nanomaterials contact with cells can induce oxidative stress reaction, allergic reaction, and then produce cytotoxicity and genetic toxicity. Therefore, the study of nano-toxicology has attracted more and more attention. Aim: to compare the cytocompatibility of pluronic (P85F127 and F87) triblock copolymers modified with folic acido FA and polylactic acid (poly (lactic acid). Methods: Pluronic triblock copolymers (P85 F127 and F87) were modified with polylactic acid and folic acid at the ends of the copolymers. A variety of amphiphilic block copolymers (PLA-P85-PLAFA-P85-PLAP-F127-APLFA-F127-PLA-F87-PLA and FA-F87-PLA) nanoparticles were synthesized. HepG-2 cells were selected to evaluate the cytotoxicity of nanoparticles in terms of cell morphology, cell metabolic activity and cell membrane effect. Results and conclusion: (1) Hep G-2 cells were treated with P85F127 and F87 nanoparticles without folic acid and polylactic acid for 24 h, compared with control group. There was no significant difference in cell relative growth rate (P0.05), and in the range of low mass concentration (510g / L), the cell proliferation was promoted. The proliferation of Hep G-2 cells was significantly inhibited by P85 and F87 nanoparticles modified by folic acid and polylactic acid (P85 / F127 and F87 nanoparticles modified by folic acid and polylactic acid) at a concentration of 400 mg / L, (2) Hep G-2 cells were treated with folic acid and poly (lactic acid) modified P85 / F127 and F87 nanoparticles (5 / 10 / L / L, 50 / 100, 200400 mg / L), compared with the control group. There was no significant difference (P0.05); (3). The results showed that the PLA-Pluronic-PLA nanoparticles and FA-Pluronic-PLA nanoparticles could be used as drug carriers when modified with folic acid and poly (lactic acid) triblock copolymers (P85 F127 and F87).
【作者单位】： 江西科技师范大学生命科学学院;
【基金】：国家自然科学基金项目(31360376,21264009) 江西省主要学科学术和技术带头人培养计划(20153BCB 22009) 江西省高等学校科技落地计划项目(KJLD13071)~~
【分类号】：R99;TB383.1
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