载银磷酸钙纳米线复合树脂的制备与表征
发布时间：2018-07-27 16:46
【摘要】：随着计算机辅助设计和计算机辅助制造技术(CAD/CAM)在口腔修复中的广泛应用,与CAD/CAM系统配套的各类可切削材料也逐渐成为研究者关注的对象,相比于陶瓷和金属材料,可切削复合树脂块用于制造个性化临时或长期使用修复体具有明显优势。本论文首先通过水热法合成了具有一维结构的羟基磷灰石(HA)纳米线,对其进行表面改性后,得到与丙烯酸酯树脂具有良好界面粘结的载银HA纳米线；将该HA纳米线作为功能填料,添加到齿科修复常用双酚A甲基丙烯酸缩水甘油酯(Bis-GMA)树脂中,固化后得到一种具有抑菌效果的功能性复合树脂,可用作可切削材料。主要工作如下：1、以油酸钙和磷酸二氢钠为原料,探索水热反应温度和时间,在优化反应条件(180℃,23h)下,得到一维HA纳米线；并通过多巴胺的氧化自聚合完成对该HA纳米线的表面包覆改性2、 借助多巴胺的粘附性和聚乙烯吡咯烷酮的助分散作用,采用吐伦试剂(即AgNO3的氨水溶液),在HA纳米线表面负载均匀分散的银纳米粒子。调节AgNO3的浓度,可控制在HA纳米线表面负载的银纳米粒子含量。在不同的银纳米粒子负载量下,载银HA纳米纤维及其浸提液都表现出对变形链球菌(S.M.)的良好抑菌性；细胞毒性评价实验显示,当浸提液中的银离子浓度低于1.6ppm时,对L929成纤维细胞没有表现出明显的细胞毒性。比较抑菌实验和细胞毒性实验结果发现,在两者之间存在一定的银离子浓度范围(1.0-1.6ppm),使材料既具有抑菌性,又没有明显细胞毒性。3、选取负载合适银纳米粒子的HA纳米线,以不同比例添加到Bis-GMA/TEGDMA (w:w,1:1)树脂中,加入过氧化二苯甲酰(BPO)为引发剂,于120℃下热固化12 h,得到复合树脂样品。扫描电镜观察和三点弯力学性能检测显示,当HA纳米线含量在6 wt.%-8 wt.%之间时,纳米线在树脂基体中分散良好,界面粘结性好,相比于纯树脂固化物,复合树脂的弯曲强度提高了约20%。在纯树脂片和含不同载银HA纳米线复合树脂片表面接种S.M.,培养S.M.24 h后进行live/dead染色,激光共聚焦显微镜下观察显示,纯树脂片没有抑菌效果,而在含载银HA纳米线复合树脂片的S.M.已经大量死亡,表现出好的接触抑菌效果。从含载银HA纳米线树脂片的银离子释放检测结果,结合前述的细胞毒性评价,可以确认,这些含载银HA纳米线复合树脂在口腔应用环境下,不会产生明显的细胞毒性问题。
[Abstract]:With the wide application of computer-aided design (CAD) and computer-aided manufacturing (CAD/CAM) technology in oral prosthesis, various kinds of machinable materials matching with CAD/CAM system have gradually become the object of attention of researchers, compared with ceramic and metal materials. The machinable composite resin block has obvious advantages in the manufacture of personalized temporary or long-term restorations. In this paper, hydroxyapatite (HA) nanowires with one-dimensional structure were synthesized by hydrothermal method. After surface modification, silver loaded HA nanowires with good interfacial bonding with acrylate resin were obtained. The HA nanowires were added to the common bisphenol A glycidyl methacrylate (Bis-GMA) resin for dental repair. After curing, a functional composite resin with bacteriostasis effect was obtained, which can be used as cutting material. The main work is as follows: 1. Using calcium oleate and sodium dihydrogen phosphate as raw materials, the hydrothermal reaction temperature and time are explored and one-dimensional HA nanowires are obtained under optimized reaction conditions (180 鈩，
本文编号：2148464
[Abstract]:With the wide application of computer-aided design (CAD) and computer-aided manufacturing (CAD/CAM) technology in oral prosthesis, various kinds of machinable materials matching with CAD/CAM system have gradually become the object of attention of researchers, compared with ceramic and metal materials. The machinable composite resin block has obvious advantages in the manufacture of personalized temporary or long-term restorations. In this paper, hydroxyapatite (HA) nanowires with one-dimensional structure were synthesized by hydrothermal method. After surface modification, silver loaded HA nanowires with good interfacial bonding with acrylate resin were obtained. The HA nanowires were added to the common bisphenol A glycidyl methacrylate (Bis-GMA) resin for dental repair. After curing, a functional composite resin with bacteriostasis effect was obtained, which can be used as cutting material. The main work is as follows: 1. Using calcium oleate and sodium dihydrogen phosphate as raw materials, the hydrothermal reaction temperature and time are explored and one-dimensional HA nanowires are obtained under optimized reaction conditions (180 鈩，
本文编号：2148464
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