掺硒羟基磷灰石生物材料的制备、表征及其性能的研究

发布时间：2018-10-18 10:07

【摘要】：本论文以水热合成法制备的不同硒掺杂的纳米羟基磷灰石(SeHAp)和SeHAp的体外蛋白药物负载与释放为研究重点,同时对其生物活性进行了评价。首先,以Ca(NO3)2·4H2O、(NH4)2HPO4和Na2SeO3分别作为钙源、磷源和硒源,利用水热法在160°C水热反应36 h合成了具有药物缓释性能和生物活性的SeHAp。采用X射线衍射仪(XRD)、傅利叶变换红外光谱仪(FT-IR)、扫描电子显微镜(SEM)、电子衍射光谱仪(EDX)、透射电子显微镜(TEM)、热重-差示扫描量热分析(TG-DSC)、化学滴定分析法和纳米粒度及Zeta电位仪对产品进行了表征。结果表明当掺杂量n(Se):n(P)0.5时,所合成的纳米SeHAp为均相,且随着硒含量的增大样品的结晶度和晶粒呈递减的趋势,SeO32 通过取代PO43 全部的掺杂到羟基磷灰石中;SeHAp在模拟体液(SBF)中呈正电性且带电量随硒含量的增加呈递减趋势。其次,选用蛋白药物牛血清白蛋白(BSA)作为研究模型,对SeHAp吸附/释放性能进行了研究与分析。研究发现SeHAp通过C位点对BSA进行吸附,吸附过程符合朗格缪尔吸附等温式且吸附量随着掺杂硒磷摩尔比的增大而减小。SeHAp-BSA在SBF中释放72 h,其释放过程分为两个阶段:在最初的12 h,样品均出现突释的现象,随后表现为缓释的性能。最后,采用体外模拟实验法对材料的生物活性进行了科学合理的评价。结果表明将材料浸泡在SBF中,其将会同时进行生物降解和新骨的生成,因此证实了SeHAp具有较好的生物活性。
[Abstract]:In this paper, the in vitro protein drug loading and release of different selenium doped hydroxyapatite (SeHAp) and SeHAp (hydroxyapatite) prepared by hydrothermal synthesis were studied, and their biological activities were evaluated. Firstly, using Ca (NO3) 24H _ 2O, (NH4) 2HPO4 and Na2SeO3 as calcium, phosphorus and selenium sources, SeHAp. with sustained release and biological activity was synthesized by hydrothermal reaction at 160 掳C for 36 h. X-ray diffractometer (XRD), Fourier transform infrared spectrometer (FT-IR), scanning electron microscope (SEM), electron diffraction spectrometer, (EDX), transmission electron microscope (TEM), thermogravimetric differential scanning calorimetry (TG-DSC), chemical titration analysis, nano-particle size and Zeta were used. The product was characterized by potentiometer. The results show that when the doping amount of n (Se): n (P) is 0.5, the synthesized SeHAp is homogeneous. With the increase of selenium content, the crystallinity and grain size of the sample decreased, SeO32 doped into hydroxyapatite by replacing PO43, and SeHAp showed positive electrical property in simulated body fluid (SBF) and the electric energy decreased with the increase of selenium content. Secondly, the adsorption / release properties of bovine serum albumin (BSA) were studied and analyzed by using bovine serum albumin (BSA) as the model. It was found that SeHAp adsorbs BSA through C-site. The adsorption process was consistent with Langmuir adsorption isotherm and the adsorption capacity decreased with the increase of the molar ratio of doped selenium and phosphorus. The release of SeHAp-BSA in SBF for 72 h was divided into two stages: at the first 12 h, the samples showed sudden release. Then the performance of slow release. Finally, the biological activity of the material was evaluated scientifically and reasonably by in vitro simulation experiment. The results showed that when the material was immersed in SBF, it would be biodegradable and new bone formation at the same time. Therefore, it was proved that SeHAp had better bioactivity.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ460.1;TB383.1

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