基于金纳米簇多功能纳米粒子的合成及应用研究
发布时间：2018-11-26 12:59
【摘要】：纳米粒子是指粒度在1-100 nm之间的粒子,它具有表面效应、量子尺寸效应、小尺寸效应及宏观量子隧道效应,并由此派生出传统固体不具有的许多特殊性质,导致纳米粒子的力、磁、光、声、热、电等性能与宏观材料的特性有明显的不同。因而,对纳米材料的制备与应用的研究已成为当今材料科学研究领域的热点。近年来,金纳米簇(AuNCs)作为一种新型的荧光纳米材料在癌症诊断和治疗方面的应用已经逐渐受到研究人员的重视。与传统荧光材料如量子点、有机染料等相比,AuNCs有毒性低、化学和光学稳定性好、荧光寿命长、荧光强度大等优点。基于AuNCs的良好的生物相容性和易于合成的优点,AuNCs作为新型荧光试剂在生物医学领域的研究中越来越受到关注。由于可以克服单一成像模式的弊端、集合单一成像的优点,多功能成像纳米探针的发展在癌症诊疗中具有日趋重要的意义。本文首次提出一种新型、简便的方法,将具有聚集增强荧光效应的Gd3+诱导聚集的AuNCs聚集体包覆于二氧化硅(Si02)壳中制备出形貌均一、分散性良好的Gd3+-A-AuNCs@Si02纳米粒子(荧光强度为同浓度AuNCs的3.8倍)。同时,由于以Gd3+为基础的复合物可用于核磁(MR)成像,含Au的纳米粒子可用于计算机X射线断层扫描(CT)成像,因此制备出的纳米粒子具有作为多功能成像纳米探针的潜质。经过实验,合成的纳米粒子在小鼠体内和体外多功能(荧光/CT/MR)成像方面具有良好的应用潜能。进一步工作中,我们用一种简便、通用的以聚丙烯酸(PAA)为模板的方法,通过将双功能(CT/荧光)成像的核包覆,得到了蛋黄-蛋壳结构的A-AuNC@PAA/mSiO2纳米粒子,外层的Si02壳中的堆积孔同时赋予了蛋黄-蛋壳结构纳米粒子运载药物的能力。制备出的纳米粒子可用于体内和体外双功能成像,也可用作pH响应药物载体,对肿瘤细胞进行化学治疗,实现了癌症治疗中的诊断、治疗一体化。
[Abstract]:Nanoparticles are particles with a particle size of 1-100 nm. They have surface effect, quantum size effect, small size effect and macroscopic quantum tunneling effect, and derive many special properties that traditional solids do not have. The mechanical, magnetic, optical, acoustic, thermal and electrical properties of the nanoparticles are obviously different from those of the macroscopic materials. Therefore, the preparation and application of nanomaterials have become a hot spot in the field of material science. In recent years, the application of gold nanocluster (AuNCs) as a new fluorescent nano-material in cancer diagnosis and treatment has been paid more and more attention by researchers. Compared with traditional fluorescent materials such as quantum dots and organic dyes, AuNCs has the advantages of low toxicity, good chemical and optical stability, long fluorescence lifetime and high fluorescence intensity. Based on the advantages of good biocompatibility and easy synthesis of AuNCs, as a new fluorescent reagent, has attracted more and more attention in biomedical research. Because the disadvantages of single imaging mode can be overcome and the advantages of single imaging can be integrated, the development of multifunctional imaging nanoprobes is becoming more and more important in cancer diagnosis and treatment. In this paper, a new and simple method is proposed, in which the aggregates of Gd3 with agglomeration enhanced fluorescence effect are coated in silica (Si02) shells, and the morphology of AuNCs aggregates is uniform. Gd3-A-AuNCs@Si02 nanoparticles with good dispersion (fluorescence intensity 3. 8 times that of AuNCs of the same concentration). At the same time, because Gd3 based composites can be used for nuclear magnetic (MR) imaging, nanoparticles containing Au can be used in computed X-ray tomography (CT) imaging. Therefore, the prepared nanoparticles have the potential to be multifunctional imaging nanoprobes. Experimental results show that the synthesized nanoparticles have good application potential in vivo and in vitro multifunctional (fluorescence / CT/MR) imaging of mice. In further work, using a simple and general method using polyacrylic acid (PAA) as template, we obtained A-AuNC@PAA/mSiO2 nanoparticles with the structure of yolk and eggshell by coating the nuclei of CT/ imaging. The stacking holes in the outer Si02 shell also give the yolk-eggshell structure the ability to deliver drugs. The prepared nanoparticles can be used in bifunctional imaging in vivo and in vitro, and can also be used as pH response drug carrier for chemotherapy of tumor cells.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O614.123
[Abstract]:Nanoparticles are particles with a particle size of 1-100 nm. They have surface effect, quantum size effect, small size effect and macroscopic quantum tunneling effect, and derive many special properties that traditional solids do not have. The mechanical, magnetic, optical, acoustic, thermal and electrical properties of the nanoparticles are obviously different from those of the macroscopic materials. Therefore, the preparation and application of nanomaterials have become a hot spot in the field of material science. In recent years, the application of gold nanocluster (AuNCs) as a new fluorescent nano-material in cancer diagnosis and treatment has been paid more and more attention by researchers. Compared with traditional fluorescent materials such as quantum dots and organic dyes, AuNCs has the advantages of low toxicity, good chemical and optical stability, long fluorescence lifetime and high fluorescence intensity. Based on the advantages of good biocompatibility and easy synthesis of AuNCs, as a new fluorescent reagent, has attracted more and more attention in biomedical research. Because the disadvantages of single imaging mode can be overcome and the advantages of single imaging can be integrated, the development of multifunctional imaging nanoprobes is becoming more and more important in cancer diagnosis and treatment. In this paper, a new and simple method is proposed, in which the aggregates of Gd3 with agglomeration enhanced fluorescence effect are coated in silica (Si02) shells, and the morphology of AuNCs aggregates is uniform. Gd3-A-AuNCs@Si02 nanoparticles with good dispersion (fluorescence intensity 3. 8 times that of AuNCs of the same concentration). At the same time, because Gd3 based composites can be used for nuclear magnetic (MR) imaging, nanoparticles containing Au can be used in computed X-ray tomography (CT) imaging. Therefore, the prepared nanoparticles have the potential to be multifunctional imaging nanoprobes. Experimental results show that the synthesized nanoparticles have good application potential in vivo and in vitro multifunctional (fluorescence / CT/MR) imaging of mice. In further work, using a simple and general method using polyacrylic acid (PAA) as template, we obtained A-AuNC@PAA/mSiO2 nanoparticles with the structure of yolk and eggshell by coating the nuclei of CT/ imaging. The stacking holes in the outer Si02 shell also give the yolk-eggshell structure the ability to deliver drugs. The prepared nanoparticles can be used in bifunctional imaging in vivo and in vitro, and can also be used as pH response drug carrier for chemotherapy of tumor cells.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O614.123
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