茉莉酸受体多功能纳米探针的合成与应用
发布时间：2019-01-08 10:45
【摘要】：将巯基丙酸修饰的CdTe量子点和植物激素茉莉酸通过一步法偶联到表面修饰氨基的Fe_3O_4纳米颗粒表面,合成了一种同时具有荧光信号、磁响应性能和特异性标记植物原生质体中茉莉酸受体的多功能磁性荧光纳米探针.荧光光谱测试表明该探针保持了CdTe量子点荧光性能,具有荧光强度高、适用体系的pH范围宽和抗光漂白性能好等优点;磁响应及磁滞回线测试充分说明该探针具有良好的超顺磁性.使用该探针对绿豆原生质体中茉莉酸受体进行成像,结果表明:该探针对茉莉酸受体具有选择性标记的功能,茉莉酸受体主要存在于原生质体膜上.该标记方法简单方便,能为茉莉酸信号转导研究提供可视化的信息.
[Abstract]:The CdTe quantum dots modified with mercapto propionic acid and the plant hormone jasmonic acid were coupled to the surface of the surface modified amino Fe_3O_4 nanoparticles by one step method to synthesize a fluorescent signal. Magnetic response and specific labeled multifunctional magnetic fluorescent nanoprobes for jasmonic acid receptors in plant protoplasts. The fluorescence spectra showed that the probe kept the fluorescence properties of CdTe quantum dots, and had the advantages of high fluorescence intensity, wide range of pH and good photobleaching resistance. The magnetic response and hysteresis loop measurements show that the probe has good superparamagnetism. The results showed that the probe could selectively label the jasmonic acid receptor, which mainly existed on the protoplast membrane. The method is simple and convenient and can provide visual information for the study of jasmonic acid signal transduction.
【作者单位】： 华南师范大学化学与环境学院;
【基金】：国家自然科学基金项目(21275056,21575043) 广州市生物医药分析化学重点实验室项目(15180001) 华南师范大学青年教师培育基金项目(14KJ08)
【分类号】：O657.3;TB383.1
本文编号：2404499
[Abstract]:The CdTe quantum dots modified with mercapto propionic acid and the plant hormone jasmonic acid were coupled to the surface of the surface modified amino Fe_3O_4 nanoparticles by one step method to synthesize a fluorescent signal. Magnetic response and specific labeled multifunctional magnetic fluorescent nanoprobes for jasmonic acid receptors in plant protoplasts. The fluorescence spectra showed that the probe kept the fluorescence properties of CdTe quantum dots, and had the advantages of high fluorescence intensity, wide range of pH and good photobleaching resistance. The magnetic response and hysteresis loop measurements show that the probe has good superparamagnetism. The results showed that the probe could selectively label the jasmonic acid receptor, which mainly existed on the protoplast membrane. The method is simple and convenient and can provide visual information for the study of jasmonic acid signal transduction.
【作者单位】： 华南师范大学化学与环境学院;
【基金】：国家自然科学基金项目(21275056,21575043) 广州市生物医药分析化学重点实验室项目(15180001) 华南师范大学青年教师培育基金项目(14KJ08)
【分类号】：O657.3;TB383.1
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