可吸收引导骨再生膜力学性能研究
发布时间：2019-04-24 03:48
【摘要】：引导骨再生(Guided Bone Regeneration, GBR)是近年来新诞生的一种组织工程学方法。电纺丝是简捷连续制备一维纳米纤维技术,脂肪族聚酯已经通过静电纺丝成功地制成纳米纤维,并且在引导骨再生研究领域展现出广阔前景。然而电纺纳米纤维膜较低的力学强度成了难解的问题。为此,在本文中,我们合成了各种星型已内酯(star-shaped PCL),探讨其相关性能；将其作为增强剂与聚丙交酯共混纺丝,研究共混纤维膜的力学和相关性能的变化；还将SAPCL、改性接枝羟磷灰石(HA-g-PLLA)和表面活性剂PF-108联合,研究协同作用对纤维膜的力学及相关性能的影响；在此基础上,利用溶剂汽化交联进一步增强纤维膜。四组分复合纳米纤维膜具有良好的亲水性和力学性能适于引导骨再生修复要求。1.成功合成了2,3,4和6臂的SAPCL,通过H'-NMR、GPC、XRD、DSC对材料进行表征,结果表明相同分子量的PCLs随着臂数的增加,支臂长度逐渐变小,短的支臂更受束,它限制了多臂星型PCLs的每条臂的流动性,致使PCL的结晶度变小。2.通过静电纺丝方法成功制备了多臂PCL与聚乳酸(PLLA)共混纳米纤维膜。利用SEM观察纳米纤维的形貌,DSC研究了共混纤维膜的热学性质,并通过拉伸测试研究了SAPCL的臂数、分子量、混纺比等对纳米纤维膜的力学性能的影响,当SAPCL混入量为20wt%时,共混纤维膜的拉伸强度达到最大值,并且共混纳米纤维膜无细胞毒性。3.利用静电纺丝方法成功制备了PLGA/PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108四组分复合纤维膜。通过SEM、TEM观察了纳米纤维的形貌和无机粒子在纤维中分布,红外分析说明四组分只是物理共混,DSC研究了四组分纤维膜的热学性质,通过拉伸测试研究了不同组分对纤维膜力学性能的影响,四组分纤维膜的拉伸强度远大纯PLGA纤维膜,测试分析了不同组份纤维亲水性、降解性和生物活性的差异。其中加入PF108后,PLGA中的-CH3和PF-108中的-CH3疏水性相互作用限制EO融入PLGA聚合物链,迫使它们突向表面,使共混纤维膜的亲水性增加。MTT测试表明所获四组分纤维膜无细胞毒性。4.基于PLGA和PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108纳米纤维膜,探讨溶剂汽化交联进一步增强纳米纤维膜的力学性能。氮气流速、交联时间和交联溶剂比例是影响交联的重要因素,通过正交实验表明优化的工艺条件,并且用SEM观察交联前后的形貌变化,MTT测试表明交联纳米纤维膜无细胞毒性。
[Abstract]:Guided bone regeneration (Guided Bone Regeneration, GBR) is a new tissue engineering method in recent years. Electrospinning is a simple and continuous one-dimensional nanofiber preparation technology. Aliphatic polyester has been successfully made into nanofibers by electrospinning, and has shown a broad prospect in the research field of guided bone regeneration. However, the low mechanical strength of electrospun nanofiber film becomes a difficult problem. Therefore, in this paper, we synthesized various star caprolactone (star-shaped PCL), used it as reinforcing agent and polylactide blend spinning to study the change of mechanical and related properties of blend fiber membrane. SAPCL, modified hydroxyapatite (HA-g-PLLA) and surfactant PF-108 were combined to study the influence of synergistic action on the mechanical and related properties of the fiber membrane, and on the basis of this, the fiber membrane was further strengthened by solvent vaporization cross-linking. The four-component composite nanofiber membrane has good hydrophilicity and mechanical properties suitable for guiding bone regeneration and repair. 1. The SAPCL, of 2,3,4 and 6 arms were synthesized and characterized by PCLs, GPC, XRD and DSC. The results showed that the length of the arms of the same molecular weight became smaller with the increase of the number of arms, and the shorter arms were more affected by the beam, and the results showed that the PCLs of the same molecular weight gradually decreased with the increase of the number of arms. It limits the mobility of each arm of the multi-arm star-shaped PCLs and reduces the crystallinity of PCL. Multi-arm PCL / polylactic acid (PLLA) blend nanofiber membranes were successfully prepared by electrospinning. The morphology of nanofibers was observed by SEM, the thermal properties of blend fiber membranes were studied by DSC, and the effects of the number of arms, molecular weight and blending ratio of SAPCL on the mechanical properties of nano-fiber membranes were studied by tensile test. When the mixing amount of SAPCL was 20 wt%, the mechanical properties of nano-fiber membranes were studied. The tensile strength of the blend fiber membrane reaches the maximum and the blend nanofiber membrane has no cytotoxicity. 3. PLGA/20%SAPCL/5%HA-g-PLLA/1%PF- 108 four-component composite fiber membrane was successfully prepared by electrospinning. The morphology of nano-fiber and the distribution of inorganic particles in the fiber were observed by SEM,TEM. The infrared analysis showed that the four components were only physical blend. The thermal properties of the four-component fiber membrane were studied by DSC. The effects of different components on the mechanical properties of fiber membrane were studied by tensile test. The tensile strength of four-component fiber membrane was much larger than that of pure PLGA fiber membrane. The differences of hydrophilicity, degradability and biological activity of four-component fiber membrane were tested and analyzed. With the addition of PF108, the hydrophobic interaction between-CH3 in PLGA and-CH3 in PF-108 restricts EO's inclusion in the PLGA polymer chain, forcing them to protrude to the surface. The hydrophilicity of the blend fiber membrane was increased. MTT test showed that the four components of the blend fiber membrane had no cytotoxicity. 4. Based on PLGA and PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108 nano-fiber membrane, the mechanical properties of nano-fiber membrane were further enhanced by solvent vaporization cross-linking. Nitrogen flow rate, cross-linking time and cross-linking solvent ratio are important factors to influence cross-linking. The optimum technological conditions are shown by orthogonal experiment, and the morphology changes before and after cross-linking are observed by SEM. MTT test shows that the cross-linked nano-fiber membrane has no cytotoxicity.
【学位授予单位】：长春工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.2
本文编号：2464079
[Abstract]:Guided bone regeneration (Guided Bone Regeneration, GBR) is a new tissue engineering method in recent years. Electrospinning is a simple and continuous one-dimensional nanofiber preparation technology. Aliphatic polyester has been successfully made into nanofibers by electrospinning, and has shown a broad prospect in the research field of guided bone regeneration. However, the low mechanical strength of electrospun nanofiber film becomes a difficult problem. Therefore, in this paper, we synthesized various star caprolactone (star-shaped PCL), used it as reinforcing agent and polylactide blend spinning to study the change of mechanical and related properties of blend fiber membrane. SAPCL, modified hydroxyapatite (HA-g-PLLA) and surfactant PF-108 were combined to study the influence of synergistic action on the mechanical and related properties of the fiber membrane, and on the basis of this, the fiber membrane was further strengthened by solvent vaporization cross-linking. The four-component composite nanofiber membrane has good hydrophilicity and mechanical properties suitable for guiding bone regeneration and repair. 1. The SAPCL, of 2,3,4 and 6 arms were synthesized and characterized by PCLs, GPC, XRD and DSC. The results showed that the length of the arms of the same molecular weight became smaller with the increase of the number of arms, and the shorter arms were more affected by the beam, and the results showed that the PCLs of the same molecular weight gradually decreased with the increase of the number of arms. It limits the mobility of each arm of the multi-arm star-shaped PCLs and reduces the crystallinity of PCL. Multi-arm PCL / polylactic acid (PLLA) blend nanofiber membranes were successfully prepared by electrospinning. The morphology of nanofibers was observed by SEM, the thermal properties of blend fiber membranes were studied by DSC, and the effects of the number of arms, molecular weight and blending ratio of SAPCL on the mechanical properties of nano-fiber membranes were studied by tensile test. When the mixing amount of SAPCL was 20 wt%, the mechanical properties of nano-fiber membranes were studied. The tensile strength of the blend fiber membrane reaches the maximum and the blend nanofiber membrane has no cytotoxicity. 3. PLGA/20%SAPCL/5%HA-g-PLLA/1%PF- 108 four-component composite fiber membrane was successfully prepared by electrospinning. The morphology of nano-fiber and the distribution of inorganic particles in the fiber were observed by SEM,TEM. The infrared analysis showed that the four components were only physical blend. The thermal properties of the four-component fiber membrane were studied by DSC. The effects of different components on the mechanical properties of fiber membrane were studied by tensile test. The tensile strength of four-component fiber membrane was much larger than that of pure PLGA fiber membrane. The differences of hydrophilicity, degradability and biological activity of four-component fiber membrane were tested and analyzed. With the addition of PF108, the hydrophobic interaction between-CH3 in PLGA and-CH3 in PF-108 restricts EO's inclusion in the PLGA polymer chain, forcing them to protrude to the surface. The hydrophilicity of the blend fiber membrane was increased. MTT test showed that the four components of the blend fiber membrane had no cytotoxicity. 4. Based on PLGA and PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108 nano-fiber membrane, the mechanical properties of nano-fiber membrane were further enhanced by solvent vaporization cross-linking. Nitrogen flow rate, cross-linking time and cross-linking solvent ratio are important factors to influence cross-linking. The optimum technological conditions are shown by orthogonal experiment, and the morphology changes before and after cross-linking are observed by SEM. MTT test shows that the cross-linked nano-fiber membrane has no cytotoxicity.
【学位授予单位】：长春工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.2
【参考文献】
相关期刊论文 前1条
1 张鹏云;张鹏云;张鹏云;张建松;张建松;张建松;徐晓红;徐晓红;莫秀梅;莫秀梅;莫秀梅;莫秀梅;何创龙;何创龙;;生物交联剂京尼平对静电纺明胶纳米纤维膜改性的影响[J];中国组织工程研究与临床康复;2009年08期
，本文编号：2464079
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