基于介孔二氧化钛复合材料拉曼免疫传感器的研究及介孔二氧化锡光电性质初探

发布时间：2019-05-23 04:34

【摘要】：拉曼散射光谱是一种十分重要的检测手段,具有耗样量少、检测速度快、谱图容易识别等特点。采用这种方法可以直接进行无损伤的定性或者定量分析,操作也十分方便。拉曼散射光谱主要应用于生物分子的检测、催化剂结构分析等领域。目前将拉曼散射光谱和生化分析结合起来,利用拉曼散射光谱的高灵敏性和免疫反应的高特异性制备以拉曼光谱为基础的免疫传感器是近年来研究的热点。同时本文对介孔SnO2薄膜的光电性质进行了探讨,本文主要研究内容为:1.建立了一种基于纳米介孔二氧化钛和纳米银颗粒复合材料的拉曼免疫传感器,利用透射电镜(TEM)、扫描电镜(SEM)、XRD衍射、UV-Vis、拉曼光谱等对材料进行了表征。实验中利用二氧化钛与银纳米粒子协同增强对巯基苯甲酸拉曼分子信号的原理,利用“三明治”形式的双抗夹心法,通过免疫作用将甲胎蛋白一抗、甲胎蛋白抗原、标记有拉曼信标分子的二抗依次接到磁珠上,磁性分离后用于拉曼检测。实现了对甲胎蛋白(AFP)的定量检测。检测AFP抗原的线性范围是0.01 ng/mL~0.6 ng/mL,检测限是0.005 ng/m L。2.构建了一种基于纳米介孔二氧化钛和纳米金银复合材料的拉曼免疫传感器,利用透射电镜(TEM)、扫描电镜(SEM)、XRD衍射、UV-Vis、拉曼光谱等对材料进行了表征。实验中利用二氧化钛与金银复合材料协同增强对巯基苯甲酸拉曼分子信号的原理,利用“三明治”形式的双抗夹心法,通过免疫作用将甲胎蛋白一抗、甲胎蛋白抗原、标记有拉曼信标分子的二抗依次接到磁珠上,磁性分离后用于拉曼检测。实现了对甲胎蛋白(AFP)的定量检测。检测AFP抗原的线性范围是0.001 ng/mL~0.4 ng/mL,检测限是0.0005 ng/mL。3.采用溶胶-凝胶法制备纳米介孔二氧化锡,并利用TEM,SEM,XRD等分析手段对纳米介孔二氧化锡进行了表征,对ITO/SnO2电极进行光电信号的检测,研究了草酸钾,三乙醇胺两种电子供体和CdS量子点对电极的光电行为的影响。发现介孔二氧化锡薄膜在沉积了量子点后的光电信号最高,能够达到1.4A,在三乙醇胺的PBS溶液中次之,能够达到1.1 A,在PBS的缓冲溶液中最弱,只有0.17A左右。证明了电子给体三乙醇胺与Cd S量子点对介孔SnO2薄膜具有明显的光电增强作用。
[Abstract]:Raman scattering spectroscopy is a very important detection method, which has the characteristics of low sample consumption, fast detection speed, easy recognition of spectrum and so on. By using this method, the qualitative or quantitative analysis without damage can be carried out directly, and the operation is also very convenient. Raman scattering spectroscopy is mainly used in biomolecules detection, catalyst structure analysis and other fields. At present, the combination of Raman scattering spectrum and biochemical analysis, using the high sensitivity of Raman scattering spectrum and the high specificity of immune response, the preparation of immunosensors based on Raman spectroscopy is a hot research topic in recent years. At the same time, the photoelectric properties of mesoporous SnO2 thin films are discussed in this paper. the main contents of this paper are as follows: 1. A Raman biosensor based on nano-mesoporous titanium dioxide and silver nanoparticles was established. The materials were characterized by transmission electron microscope (TEM),) scanning electron microscope (SEM), XRD) diffraction and UV-Vis, Raman spectroscopy. In the experiment, the Raman signal of p-mercaptobenzoate was enhanced by titanium dioxide and silver nanoparticles, and the first antibody of alpha-fetoprotein and alpha-fetoprotein antigen were immunized by sandwich double antibody sandwich method. The second antibodies marked with Raman beacons are connected to the magnetic beads in turn and used for Raman detection after magnetic separation. The quantitative detection of alpha-fetoprotein (AFP) was realized. The linear range of detection of AFP antigen was 0.01ng/mL~0.6 ng/mL, and the detection limit was 0.005 ng/m L. 2. A Raman biosensor based on nano-mesoporous titanium dioxide and nano-gold-silver composites was constructed and characterized by transmission electron microscope (TEM),) scanning electron microscope (SEM), XRD) diffraction and UV-Vis, Raman spectroscopy. In the experiment, the Raman signal of p-mercaptobenzoate was enhanced by titanium dioxide and gold and silver composites, and the first antibody of alpha-fetoprotein and alpha-fetoprotein antigen were immunized by sandwich double antibody sandwich method. The second antibodies marked with Raman beacons are connected to the magnetic beads in turn and used for Raman detection after magnetic separation. The quantitative detection of alpha-fetoprotein (AFP) was realized. The linear range of detection of AFP antigen is 0.001 ng/mL~0.4 ng/mL,. The detection limit is 0.0005 ng/mL.3.. Nano-mesoporous tin dioxide was prepared by sol-gel method, and the nano-mesoporous tin dioxide was characterized by TEM,SEM,XRD and other analytical methods. The photoelectric signal of ITO/SnO2 electrode was detected, and potassium oxalate was studied. Effects of triethanolamine two electron donors and CdS quantum dots on the photoelectric behavior of the electrode. It is found that the photoelectric signal of mesoporous tin dioxide thin films is the highest after the deposition of quantum dots, which can reach 1.4A, the second in PBS solution of triethanolamine, 1.1 A, and the weakest in the buffer solution of PBS, only about 0.17A. It is proved that the electron donor triethanolamine and Cd S quantum dots have obvious photoelectric enhancement effect on mesoporous SnO2 thin films.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.37;TB33

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