基于多功能二硫化钼纳米载体的肿瘤诊断治疗一体化研究
发布时间：2021-10-29 01:09
恶性肿瘤分为癌和肉瘤,由于其具有无限增殖、易转移、浸润性强的特点,成为医学上的难关之一,逐年攀升的发病率和死亡率严重危害着病人的健康和生命。仅2017年就有新发癌症病例168.9万例,死亡病例60.6万例,相当于每分钟至少有8人死于癌症。肿瘤已成为人类健康和生命的重大威胁,因此早期诊断和规范的治疗显得尤为重要。临床上常用的治疗肿瘤的手段有手术治疗、化学药物治疗、放射治疗治疗。手术作为常见的一种治疗方法,对大多数早期原发性病灶治疗有较好的效果,但对于中、晚期恶性肿瘤的治疗效果并不理想,并且手术治疗对人体还会造成较大的创伤,导致患者的免疫力下降,非常容易引起术后一系列并发症。放射治疗是指利用不同能量的放射性射线照射肿瘤细胞,从而达到杀伤人体病灶部位的肿瘤细胞的作用。但是,放射性治疗的疗效受限于肿瘤组织对放射线的敏感程度,并且在放射性射线杀灭和消除肿瘤细胞的同时,也会对其人体周围的健康组织细胞造成一定的损伤。化学药物治疗是一种全身性的治疗方法,但是其选择性不好,并且长期使用抗肿瘤化学治疗药物可能会诱发肿瘤细胞的耐药性等问题。由于三大传统治疗手段的临床效果受到限制,近年来新兴的具有非侵入、无创...
【文章来源】：天津大学天津市 211工程院校 985工程院校 教育部直属院校
【文章页数】：96 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
Chapter 1 Introduction
1.1 Research background and objectives
1.2 Photothermal therapy (PTT)
1.2.1 Photothermal agents
1.3 Photodynamics therapy (PDT)
1.4 Combination therapy for antitumor
1.4.1 Combination of PTT and Chemotherapy for antitumor
1.4.2 Combination of PDT and Chemotherapy for antitumor
1.4.3 Combination of PTT and PDT for antitumor
1.5 Multi-modality imaging guided NIR light-induced tumor therapy
1.6 The design of the project
1.6.1 The meaning of the project
1.6.2 Content of the project
Chapter 2 Preparation and characterization of PEG-Mo S_2-Au NPs-Ce6 nanocomposites
2.1 Introduction
2.2 Experimental materials and instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Experiment section
2.3.1 Preparation of PEG-Mo S_2 nanosheets
2.3.2 Ce6 Loading capacities
2.3.3 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
2.3.4 In vitro measurement of photothermal performance
2.3.5 Drug release
2.4 Results and discussion
2.4.1 Synthesis and characterization of PEG-Mo S_2-Au-Ce6 nanocomposites
2.4.2 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
2.4.3 In vitro measurement of photothermal performance
2.4.4 Drug loading and release
2.5 Conclusion
Chapter 3 PTT/PDT effect evaluation of PEG-Mo S_2-Au-Ce6 nanocomposites in vitroand in vivo
3.1 Introduction
3.2 Experimental materials and instruments
3.2.1 Materials
3.2.2 Instruments
3.3 Experiment section
3.3.1 Cell cultured experiment
3.3.2 Cytotoxicity assay
3.3.3 In vitro cellular uptake study
3.3.4 Intracellular ROS detection
3.3.5 Animals and tumors model
3.3.6 In Vivo NIRF and CT Imaging
3.3.7 In Vivo photothermal imaging
3.3.8 In vivo antitumor activity
3.3.9 Hematoxylin and Eosin (H&E) Staining Analysis
3.3.10 Statistical analysis
3.4 Results and Discussion
3.4.1 MTT assay and Calcein AM/PI co-stained study
3.4.2 Cellular uptake study and ROS detection
3.4.3 In vivo NIRF and CT imaging
3.4.4 In vivo photothermal imaging
3.4.5 In vivo PTT/PDT for anticancer treatment
3.5 Conclusion
Chapter 4 Preparation and characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
4.1 Introduction
4.2 Materials and instruments
4.2.1Experimental material
4.2.2 Instruments
4.3 Experimental section
4.3.1 Preparation of Mo S_2 nanodots
4.3.2 Preparation of PEGlyted Mo S_2@Si O_2 nanoparticles
4.3.3 Drug loading and CS coating
4.3.4 Photothermal effect
4.3.5 Singlet oxygen detection
4.4 Results and discussion
4.4.1 Characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
4.5 Conclusion
Chapter 5 PTT/PDT effect evaluation of Alpc-Mo S_2@Si O_2-CS nanocomposites in vitroand in vivo
5.1 Introduction
5.2 Materials and instruments
5.2.1 Materials
5.2.2 Instruments
5.3 Experiment section
5.3.1 Detection of intracellular ROS
5.3.2 Cell culture
5.3.3 In vitro cytotoxicity evaluation
5.3.4 In vitro cell experiments
5.3.5 Animals and tumor models
5.3.6 NIRF, PA, CT and infrared thermal imaging and ex vivo detection ofROS
5.3.7 In vivo antitumor efficiency
5.3.8 Statistics
5.4 Results and discussion
5.4.1 In vitro cell experiments
5.4.2 In vivo NIRF/PA/CT multimodal imaging
5.4.3 In vivo antitumor activity
5.5 Conclusion
Chapter 6 Conclusion and future work
6.1 Conclusion
6.2 Innovation of this dissertation
6.3 Future work
References
Published papers and scientific description
Acknowledgments
本文编号：3463671
【文章来源】：天津大学天津市 211工程院校 985工程院校 教育部直属院校
【文章页数】：96 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
Chapter 1 Introduction
1.1 Research background and objectives
1.2 Photothermal therapy (PTT)
1.2.1 Photothermal agents
1.3 Photodynamics therapy (PDT)
1.4 Combination therapy for antitumor
1.4.1 Combination of PTT and Chemotherapy for antitumor
1.4.2 Combination of PDT and Chemotherapy for antitumor
1.4.3 Combination of PTT and PDT for antitumor
1.5 Multi-modality imaging guided NIR light-induced tumor therapy
1.6 The design of the project
1.6.1 The meaning of the project
1.6.2 Content of the project
Chapter 2 Preparation and characterization of PEG-Mo S_2-Au NPs-Ce6 nanocomposites
2.1 Introduction
2.2 Experimental materials and instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Experiment section
2.3.1 Preparation of PEG-Mo S_2 nanosheets
2.3.2 Ce6 Loading capacities
2.3.3 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
2.3.4 In vitro measurement of photothermal performance
2.3.5 Drug release
2.4 Results and discussion
2.4.1 Synthesis and characterization of PEG-Mo S_2-Au-Ce6 nanocomposites
2.4.2 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
2.4.3 In vitro measurement of photothermal performance
2.4.4 Drug loading and release
2.5 Conclusion
Chapter 3 PTT/PDT effect evaluation of PEG-Mo S_2-Au-Ce6 nanocomposites in vitroand in vivo
3.1 Introduction
3.2 Experimental materials and instruments
3.2.1 Materials
3.2.2 Instruments
3.3 Experiment section
3.3.1 Cell cultured experiment
3.3.2 Cytotoxicity assay
3.3.3 In vitro cellular uptake study
3.3.4 Intracellular ROS detection
3.3.5 Animals and tumors model
3.3.6 In Vivo NIRF and CT Imaging
3.3.7 In Vivo photothermal imaging
3.3.8 In vivo antitumor activity
3.3.9 Hematoxylin and Eosin (H&E) Staining Analysis
3.3.10 Statistical analysis
3.4 Results and Discussion
3.4.1 MTT assay and Calcein AM/PI co-stained study
3.4.2 Cellular uptake study and ROS detection
3.4.3 In vivo NIRF and CT imaging
3.4.4 In vivo photothermal imaging
3.4.5 In vivo PTT/PDT for anticancer treatment
3.5 Conclusion
Chapter 4 Preparation and characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
4.1 Introduction
4.2 Materials and instruments
4.2.1Experimental material
4.2.2 Instruments
4.3 Experimental section
4.3.1 Preparation of Mo S_2 nanodots
4.3.2 Preparation of PEGlyted Mo S_2@Si O_2 nanoparticles
4.3.3 Drug loading and CS coating
4.3.4 Photothermal effect
4.3.5 Singlet oxygen detection
4.4 Results and discussion
4.4.1 Characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
4.5 Conclusion
Chapter 5 PTT/PDT effect evaluation of Alpc-Mo S_2@Si O_2-CS nanocomposites in vitroand in vivo
5.1 Introduction
5.2 Materials and instruments
5.2.1 Materials
5.2.2 Instruments
5.3 Experiment section
5.3.1 Detection of intracellular ROS
5.3.2 Cell culture
5.3.3 In vitro cytotoxicity evaluation
5.3.4 In vitro cell experiments
5.3.5 Animals and tumor models
5.3.6 NIRF, PA, CT and infrared thermal imaging and ex vivo detection ofROS
5.3.7 In vivo antitumor efficiency
5.3.8 Statistics
5.4 Results and discussion
5.4.1 In vitro cell experiments
5.4.2 In vivo NIRF/PA/CT multimodal imaging
5.4.3 In vivo antitumor activity
5.5 Conclusion
Chapter 6 Conclusion and future work
6.1 Conclusion
6.2 Innovation of this dissertation
6.3 Future work
References
Published papers and scientific description
Acknowledgments
本文编号：3463671
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