基于阻抗分析的纸基细胞培养即时检测系统的研究

发布时间：2018-03-30 23:10

本文选题：纸基细胞培养　切入点：阻抗分析　出处：《天津大学》2014年硕士论文

【摘要】：细胞培养技术是生物里最基础和核心的技术。二维细胞培养是目前主流的生物细胞培养技术,三维细胞培养近几年也有快速的发展。相对于二维细胞培养,三维细胞培养在细胞形态、特性和培养环境方面更贴近生物实际生长状况,更有利于生物的研究,但目前三维细胞培养在技术上仍有许多问题,例如细胞数目检测数据不能量化。为解决这一问题,本课题开发一种新的三维细胞培养方式—基于滤纸的细胞培养。相比水凝胶等三维细胞培养基材,滤纸具有机械可移动、亲水性、可开发性强等优点。机械可移动性使纸基细胞培养在细胞检测方面有很大优势,滤纸的亲水性使其具有很大的开发空间。本课题利用蜡染技术在滤纸上印制疏水壁垒,形成亲水流道,制作简单快速。利用滤纸的特性开发了纸基细胞培养系统和纸基微流体细胞培养系统,通过生物实验技术对其可行性进行验证,并拓展了药物筛检的实际应用。配合纸基细胞培养系统,结合细胞的阻抗特性,开发纸基细胞培养即时检测系统。本系统利用黄光微影和金属气相沉积等技术制备检测电极,并进行COMSOL模拟仿真确认电极的有效范围,使用普林斯顿的Versa STAT 4电化学工作站进行阻抗检测和数据采集。以往的细胞生长曲线检测属于终点检测,并且耗费较长时间。而纸基细胞培养即时检测系统具有检测快速方便、非终点检测等特点。通过纸基酶联免疫分析的检测结果来检验细胞阻抗检测系统的准确性。最终证明本方法适用于纸基细胞培养的检测。
[Abstract]:Cell culture technology is the most basic and core technology in biology.Two-dimensional cell culture is the main technology of biological cell culture, and three-dimensional cell culture has a rapid development in recent years.Compared with two-dimensional cell culture, three-dimensional cell culture is closer to the actual biological growth status in cell morphology, characteristics and culture environment, but there are still many technical problems in three-dimensional cell culture.Cell count data, for example, cannot be quantified.In order to solve this problem, a new three-dimensional cell culture method-filter paper-based cell culture was developed.Compared with hydrogel and other three-dimensional cell culture materials, filter paper has the advantages of mechanical mobility, hydrophilicity and strong exploitability.Mechanical mobility makes paper cell culture have a great advantage in cell detection, and filter paper has a great development space because of its hydrophilicity.This subject uses batik technology to print hydrophobic barrier on filter paper to form hydrophilic channel and make it simple and fast.The paper based cell culture system and paper based microfluid cell culture system were developed by using the characteristics of filter paper. The feasibility of the system was verified by biological experiment technology, and the practical application of drug screening was expanded.In combination with the paper-based cell culture system and the impedance characteristics of the cell, the paper-based cell culture instant detection system was developed.In this system, the detection electrode is prepared by using the techniques of yellow light and metal vapor deposition, and the effective range of the electrode is confirmed by COMSOL simulation. The impedance detection and data acquisition are carried out by using the Versa STAT 4 electrochemical workstation in Princeton.Previous cell growth curve detection belongs to endpoint detection and takes a long time.On the other hand, the paper-based cell culture system has the advantages of rapid and convenient detection, non-terminal detection and so on.The results of paper-based enzyme-linked immunoassay (Elisa) were used to test the accuracy of the cell impedance detection system.Finally, it is proved that this method is suitable for the detection of paper-based cell culture.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：Q813.11;TM934.7

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