阵列单液滴的捕获及单细胞分析

发布时间：2018-01-28 20:02

本文关键词： 微液滴单液滴阵列单细胞　出处：《华东理工大学学报(自然科学版)》2016年05期 　论文类型：期刊论文

【摘要】：通过流动聚焦型微液滴生成装置进行油包水液滴的制备,比较了3种不同的单液滴阵列捕获芯片对单液滴的捕获效率和操作性能,选取上下开口圆形结构获得满意的单液滴捕获。进一步利用该液滴生成和捕获一体式芯片进行了液滴中单细胞的包覆、单细胞的实时染色和细胞存活状态的检测。在优化的实验条件下制备得到了粒径为75μm的单分散液滴,液滴捕获芯片中的单液滴截留率为100%,单细胞包覆率约为33%。对捕获液滴中的单细胞进行实时染色结果表明,在5 min内获得了满意的细胞核实时染色,并通过细胞死活实时染色对细胞存活状态直接进行了表征。
[Abstract]:In this paper, three kinds of single droplet array capture chips were used to prepare oil / water droplets by flow focusing micro droplet generating device, and the efficiency and operation performance of single droplet capture chip were compared. A satisfactory single droplet capture was obtained by selecting a circular structure with an upper and lower opening. Further, the single cell coating in the droplet was carried out by using the integrated chip of the droplet generation and capture. Under the optimized experimental conditions, the single dispersed droplets with the diameter of 75 渭 m were prepared, and the rejection rate of the single droplet in the droplet capture chip was 100%. The single cell encapsulation rate was about 33. The results of real-time staining of single cell in the captured droplets showed that a satisfactory nuclear real-time staining was obtained within 5 min. Cell survival was directly characterized by real-time staining.
【作者单位】：华东理工大学分析测试中心;
【基金】：国家自然科学基金青年基金(21205041)
【分类号】：Q2-33;TN492
【正文快照】： 微流控芯片技术是近年来发展非常迅速的一个交叉学科的研究方向。它将通道尺寸在10?100 的通道网络集成在一个芯片中,可以在芯片中进行极小样品体积的引入、混合和反应,并通过多种检测方式获取有用的信息。微液滴芯片技术作为微流控芯片技术的一个重要分支,在化学、生物、

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