基于智能手机和电化学方法的多元生物传感芯片

发布时间：2018-02-08 19:55

本文关键词： 电化学方法智能手机多元检测　出处：《东南大学》2017年硕士论文　论文类型：学位论文

【摘要】：伴随着人们对健康问题的重视和现有医疗资源的匮乏,现场快速检测技术的研究在近年来成为一项热门的研究。电化学技术这门较为古老的学科与智能手机这一划时代性的智能设备相辅相成,为POCT检测提供了全新的思路:电化学方法可以实现快速、灵敏、简便的定量检测,智能手机为检测提供了系统化集成化的平台。研制基于电化学方法和智能手机平台的多元检测芯片,能够帮助人们以较低的成本和较少的操作来实时地了解身体状况。本文中设计了两种检测芯片和两种辅助检测装置。第一种多元检测芯片使用氧化酶电极法和开路电压法,通过氧化还原反应产生工作电极与参比电极之间的电压差,并推导浓度与电压的关系实现了体液中葡萄糖、乳酸、尿酸三种常见生化小分子的检测。第二种多元检测芯片则使用离子选择电极法和固态伪参比电极技术,在工作电极上制备离子选择性透过膜,并结合金属/聚吡咯电极实现了体液中钾离子、钠离子、钙离子三种常见离子的检测。两种芯片的结构相似:分为三层,上下层的基本材料为塑料透明胶片,中间层的材料为疏水性双面胶。芯片内有三个通道、三个对应的石墨工作电极和一个公共的银参比电极,可同时检测三种待测物。两种辅助检测设备通过AD采样获取芯片电极电势差,分别使用USB方法和蓝牙方法两种方式与智能手机通信。智能手机端通过应用软件接收数据,计算出结果显示在屏幕上。检测芯片、辅助检测装置与智能手机三者构成了朴素的现场快速检测系统,该系统成本低廉,易携带,易操作,为患者日常自我检测提供了极大的便利。
[Abstract]:With the shortage of people for health problems and the importance of the existing medical resources, research on rapid detection technology field become a hot research in recent years. The intelligent equipment of electrochemical technology and intelligent mobile phone is old subject of this epoch-making complement each other, provides a new method for POCT detection: electrochemical method can be achieved fast, sensitive, simple and quantitative detection, intelligent mobile phone provides a systematic integrated platform for the development of multi chip electrochemical detection. Detection method and intelligent mobile phone based platform, can help people with low cost and less operation to understand the real-time condition. This paper designed two kinds of detection chip and two an auxiliary detection device. The first kind of multiple detection chip using oxidase electrode method and open circuit voltage method, working electrode and a reference power through redox reaction The difference between the voltage polarity, and deduced the relation between concentration and voltage is realized in body fluid glucose, lactic acid, uric acid detection of three kinds of common biochemical molecules. Second multi detection chip using ion selective electrode method and solid pseudo reference electrode technique, preparation of ion selective membrane at the working electrode, and metal binding the fluid / PPy electrode in potassium ion, sodium ion, the detection of three kinds of common calcium ion. The structure of two kinds of chip is similar: divided into three layers, the basic material layer is a plastic transparent film, the middle layer is made of hydrophobic double-sided adhesive. The chip has three channels, graphite work three corresponding electrodes and a common silver reference electrode, the simultaneous detection of three analytes. Two kinds of auxiliary testing equipment through the AD sampling chip electrode potential, using the method of USB and Bluetooth method respectively in two ways with intelligence Intelligent mobile phone communication. Mobile phone terminal through the application software for receiving data, the calculated results are displayed on the screen. The detection chip, auxiliary detection device and intelligent mobile phone three made a simple on-site rapid detection system, the system has the advantages of low cost, easy to carry, easy to operate, provides great convenience for patients with daily self detection.

【学位授予单位】：东南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TN492

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