基于微流控的乳腺癌细胞三维培养芯片的设计与应用研究

发布时间：2018-03-18 07:07

本文选题：微流控芯片　切入点：乳腺癌细胞　出处：《浙江大学》2015年硕士论文　论文类型：学位论文

【摘要】：基于微流控芯片的细胞/组织芯片又称人体芯片(body-on-a-chip),是把一种细胞或多种细胞培养于微流控芯片通道内,模拟人体内微环境,实现细胞在体外的长期培养,可用于药物筛选,为解决传统药物筛选实验耗时长、耗费大的问题提供了一种新方法。本课题设计并制作了基于微流控的乳腺癌细胞三维培养芯片,该芯片可用于初步的药筛实验。芯片选用了无毒性,且具有良好可塑性和光透性的PDMS作为制作材料,通过多组实验对比,确定芯片结构为不带夹膜的双层结构,细胞培养池选用能减少流体剪切力影响的圆形细胞池。利用注射泵回抽培养基作为芯片的通液模式,不仅减少了气泡的产生,其回收液还可用于药效检测。在芯片的制作方法上,创新性地采用了成本低廉,制作简单并且可以快速成型的3D打印技术。芯片上的细胞培养结果表明该芯片可以很好地用于乳腺癌细胞的二维培养和三维培养。在微流控的作用下,芯片上的三维细胞可培养7天左右,直至形成腔体结构,微流控为细胞培养提供了营养输送的同时帮助排除废液。此外,当流速控制在50μl/h以下时,不同大小的流体剪切力在短期内对细胞生长影响不大,细胞生长状态类似。药筛实验中,将浓度为0.02μmol/ml紫杉醇作用于芯片细胞,24小时后的细胞荧光图像结果显示,紫杉醇对三维乳腺癌细胞生长有抑制作用。
[Abstract]:The cell / tissue chip based on microfluidic chip, also called human body-on-a-chip-chip-chip, is to culture one or more cells in microfluidic chip channels, simulate the microenvironment of human body, and realize long-term cell culture in vitro, which can be used for drug screening. In order to solve the problem of long time and high cost in traditional drug screening experiments, a novel microfluidic based three-dimensional culture chip for breast cancer cells was designed and fabricated. The chip can be used in the preliminary drug screening experiment. The chip uses PDMS, which is non-toxic and has good plasticity and light permeability, as the fabrication material. Through the comparison of many groups of experiments, it is determined that the chip structure is a double-layer structure without clip film. The cell culture pool is a circular cell pool which can reduce the effect of fluid shear stress. Using the injection pump back pumping medium as the liquid mode of the chip not only reduces the production of bubbles. The recycled liquid can also be used for drug efficacy testing. In the fabrication of the chip, the cost of the chip is innovatively adopted. The results of cell culture on the chip show that the chip can be used in two-dimensional and three-dimensional culture of breast cancer cells. The three-dimensional cells on the chip can be cultured for about 7 days until the cavity structure is formed. Microfluidic provides nutrition transport for cell culture and helps to remove waste liquid. In addition, when the flow rate is less than 50 渭 l / h, Fluid shear stress of different sizes had little effect on cell growth in a short period of time, but the cell growth state was similar. In the drug screening experiment, the fluorescence images of cells exposed to paclitaxel at a concentration of 0.02 渭 mol/ml for 24 hours showed that, Paclitaxel inhibits the growth of three dimensional breast cancer cells.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TN492;R737.9

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