基于CVD石墨烯的场效应管生物传感器及其检测系统研究

发布时间：2018-03-31 23:39

本文选题：石墨烯　切入点：生物传感器　出处：《山东师范大学》2017年硕士论文

【摘要】：石墨烯(Graphene)因其集优异的热学、力学、光学和电学性能,成为材料科学、生命科学和其他许多领域的研究热点。生物传感器以其在灵敏性、选择性、准确性、快速性、低成本以及操作性等方面的优势,已成为生化分析领域的重要分析技术[13]。由于石墨烯其易于与生物分子通过π-π堆垛作用结合的特性,在生物传感器设计领域受到极大关注。分子吸附在石墨烯表面可以调节其电荷传输或电荷沟道参杂,引起载流子浓度和载流子迁移率的变化。基于此,本文研究栅极电压调制的石墨烯场效应管(FET)生物传感器的构建、相关检测系统及其应用。首先介绍了石墨烯独特的结构和性质,总结了石墨烯的制备方法。随后介绍了生物传感器在国内外的研究进展及现状,生物传感器以其在高灵敏性、选择性、准确性、快速性、低成本以及操作性等方面的优势,已成为生化分析领域的重要分析技术。其次,设计制备了一种集成石墨烯场效应管(IGFET),栅极采用平面金(Au)膜,而漏极和源极采用氧化铟锡(ITO)电极。平面Au栅极与石墨烯FET集成,并连至一个可调恒压源产生栅极和石墨烯之间的稳定电场,从而调制沟道电导以及Ag/AgCl电极。由于石墨烯FET的沟道电导可以调制,那么可以将FET的等效电阻作为一个信号,通过一个桥接电路和一个检测放大器的信号获得。本文研究了FET的等效电阻与导电沟道宽度、栅极和石墨烯平板之间的距离、电介质浓度直接的关系,并利用IGFET用于ATP浓度检测。然后,基于石墨烯的光学和电学特性,利用石墨烯研制了一种光电双通道同时检测溶液pH值的场效应管传感器。一方面石墨烯FET表现出了清晰的pH值依赖型导电特性,可以在源极和漏极之间产生电流信号;另一方面在电解质溶液中添加pH荧光探针,实现pH值荧光检测。通过自主研发的一种双通道数据采集系统,可以同时采集电流和荧光两种信号。结果表明,利用该双通道数据采集系统获得比单一方法更加灵敏的pH值检测。
[Abstract]:Because of its excellent thermal, mechanical, optical and electrical properties, graphene has become a research hotspot in materials science, life sciences and many other fields. The advantages of low cost and maneuverability have become an important analytical technique in the field of biochemical analysis. [13] because graphene is easy to combine with biomolecules by 蟺-蟺 stacking, In the field of biosensor design, molecular adsorption on the surface of graphene can adjust its charge transport or charge channel impurity, resulting in changes in carrier concentration and carrier mobility. In this paper, the construction of graphene field effect tube (FET) biosensor with grid voltage modulation, the related detection system and its application are studied. Firstly, the unique structure and properties of graphene are introduced. The preparation methods of graphene were summarized, and the research progress and current situation of biosensors at home and abroad were introduced. Biosensors had the advantages of high sensitivity, selectivity, accuracy, rapidity, low cost and maneuverability. It has become an important analytical technique in the field of biochemical analysis. Secondly, a kind of integrated graphene field effect tube (IGFETT) is designed and fabricated. The gate is made of planar au film, while the drain electrode and the source electrode are made of indium tin oxide (ITO) electrode. The planar au gate is integrated with graphene FET, and the planar au gate is integrated with graphene FET. Connected to a tunable constant voltage source to generate a stable electric field between the grid and graphene, thus modulating the channel conductance and the Ag/AgCl electrode. Since the channel conductance of the graphene FET can be modulated, the equivalent resistance of the FET can be used as a signal. A bridge circuit and a detection amplifier are used to obtain the signal. The direct relationship between the equivalent resistance of FET and the width of the conductive channel, the distance between the gate and the graphene plate, and the dielectric concentration are studied. Then, based on the optical and electrical properties of graphene, A field effect tube sensor for simultaneous detection of pH value in solution by using graphene has been developed. On the one hand, graphene FET exhibits a clear pH dependent conductivity, which can generate current signals between the source and drain electrode. On the other hand, pH fluorescence probe is added to electrolyte solution to realize pH fluorescence detection. Through a dual channel data acquisition system developed by ourselves, both current and fluorescence signals can be collected simultaneously. The two-channel data acquisition system is used to obtain more sensitive pH detection than the single method.
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TN386;TP212.3

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