集成印刷电子与纸微流控装置用于床旁快速检测

发布时间：2018-04-29 14:38

  本文选题：床旁快速检测 + 心肌肌钙蛋白I　； 参考：《南京邮电大学》2015年硕士论文

【摘要】：床旁快速诊断具有快速、操作简单和节约综合成本等优点,因而广泛应用在诊所和患者家中。为了增加检测设备的便携性,Whitesides等人提出了基于纸为基底的纸质微流控芯片,具有低成本、低检测背景、较好的生物相容性、易于折叠和后处理方法简单等优点。心肌肌钙蛋白I作为理想的心脏标志物,目前临床检测主要为中心实验室检测:需要大型检测仪器和专业人员,不适合临床快速诊断。而基于纸质微流控器件的床旁快速诊断满足检测要求。基于以上背景,本论文提出一种基于喷墨印刷技术制作纸质微流控器件的方法,且利用制作的纸质微流控器件用于c Tn I的床旁快速检测。在图案化的纸质微流控芯片上,利用喷墨打印技术沉积捕获抗体,制作全打印生物检测器件。基本原理是荧光PS纳米微球作为标记物,利用荧光显微镜获得检测区域荧光信号,计算得到c Tn I的定量结果。本论文通过研究,得到最佳抗体配对为m Ab1-16A11与m Ab2-19C7,甲苯与PDMS按质量比10:1进行混合得到的溶液满足喷墨打印机的要求,最终结果表明这种纸质微流控检测器件可以用于进行生物检测,且能达到1 ng/m L的检测限。本论文还研究银电极的制作与优化:通过研究在NC膜上喷墨打印银纳米粒子制备银电极的几个重要影响因素:基板温度、点间距、打印层数和后处理时间得出,当基板温度为40℃,点间距为20μm,后处理时间为90分钟时,得到的银电极具有较小的电阻值,可以与纸质微流控器件结合进行电化学检测。
[Abstract]:Rapid bedside diagnosis has the advantages of fast operation, simple operation and cost saving, so it is widely used in clinics and patients' homes. In order to increase the portability of the detection equipment, Whitesides et al proposed a paper microfluidic chip based on paper substrate, which has the advantages of low cost, low detection background, good biocompatibility, easy folding and simple post-processing. Cardiac troponin I is an ideal cardiac marker. At present, the clinical detection is mainly a central laboratory test. It needs large detection instruments and professionals, so it is not suitable for rapid clinical diagnosis. The bed-side rapid diagnosis based on paper microfluidic device can meet the requirement of detection. Based on the above background, this paper presents a method to fabricate paper microfluidic devices based on inkjet printing technology, and makes use of the paper microfluidic devices for fast bedside detection of c TN I. In a patterned paper microfluidic chip, an all-print biological detection device was fabricated by using inkjet printing technology to deposit and capture antibodies. The basic principle is that fluorescent PS nanospheres are used as markers, fluorescence microscope is used to obtain fluorescence signal of detection region, and quantitative results of cTnI are calculated. In this paper, the best antibody pairing is m Ab1-16A11 and mAb2-19C7. The mixture of toluene and PDMS meets the requirements of inkjet printer at 10:1 mass ratio. The results show that the paper microfluidic detection device can be used for biological detection, and the detection limit of 1 ng/m L can be reached. In this paper, we also study the fabrication and optimization of silver electrode: by studying several important factors that affect the preparation of silver electrode by ink jet printing silver nanoparticles on NC film: substrate temperature, point spacing, number of printing layers and post-processing time. When the substrate temperature is 40 鈩，

本文编号：1820357