基于有机电化学晶体管的miRNA24生物传感研究

发布时间：2018-05-05 12:16

  本文选题：有机电化学晶体管 + 生物传感器　； 参考：《湖南师范大学》2016年硕士论文

【摘要】：MicroRNAs(miRNAs)是一种与生物代谢息息相关的小分子RNA。近年来,研究发现多种mi RNAs与肿瘤的生长、转移等密切相关,因此,mi RNAs的检测对于疾病,尤其是肿瘤的早期诊断具有重要意义。电化学生物传感器具有操作简便、灵敏度高以及响应速度快等很多优点,被广泛应用于miRNAs的检测中。本论文制备了基于PEDOT:PSS活性层的有机电化学晶体管(Organic Electrochemical Transistors,OECTs),利用不同的修饰电极作为OECTs的栅电极,对其相关性能进行了研究,并以此OECTs为信号放大装置,构建了适于miRNAs检测的电化学生物传感器。在最优的实验条件下,所制备的有机电化学晶体管生物传感器对miRNA24有高灵敏度、低检测下限,具有一定的应用前景。主要工作如下:(1)利用丝网印刷技术制备了碳源、漏电极,利用旋涂法制备了基于PEDOT:PSS活性层的OECTs,以HS-DNA(与mi RNA24互补)修饰的金电极作为栅电极,构建了基于有机电化学晶体管的mi RNA24生物传感器。在最优化检测条件下,该传感器对mi RNA24的检测下限为20 pM;线性范围为1 nM-200 nM;灵敏度为4.903nM-1;选择性良好,miRNA29及mismatch miRNA24对该传感器的响应较小。(2)通过Au-S键在金电极表面固定上HS-DNA,并以此为OECTs的栅电极,构建了基于亚铁氰化钾探针信号放大的有机电化学晶体管miRNA24生物传感器,并对其性能进行了详细研究。在最优化检测条件下,该传感器对miRNA24的检测下限为30 fM;线性范围为10 pM-10 nM;灵敏度为0.05989 decade-1。(3)以基于碳浆电极和Au/Ti电极的OECTs器件为模型,对OECTs器件的传输性能、输出性能、稳定性等方面进行了详细研究。此外,为了提高传感器的灵敏度,我们在栅电极上电沉积纳米金,构建了基于纳米金的miRNA24生物传感器,并对该生物传感器检测mi RNA24的可行性进行了初步研究。
[Abstract]:MicroRNAs (miRNAs) is a small molecule RNA. related to biological metabolism in recent years. It has been found that a variety of MI RNAs is closely related to the growth and metastasis of tumor. Therefore, the detection of MI RNAs is of great significance for the early diagnosis of diseases, especially the tumor. The electrochemical biosensor has the advantages of simple operation, high sensitivity and response. A lot of advantages, such as fast speed and so on, are widely used in the detection of miRNAs. In this paper, the Organic Electrochemical Transistors (OECTs) based on the active layer of PEDOT:PSS was prepared, and the different modified electrodes were used as the gate electrode of OECTs, and the correlation energy was studied, and OECTs was used as a signal amplification device. The electrochemical biosensor suitable for miRNAs detection is built. Under the optimal experimental conditions, the electrochemical transistor biosensor has high sensitivity and low detection limit for miRNA24. The main work is as follows: (1) the carbon source and leakage electrode have been prepared by the screen printing technology. Based on PEDOT:PSS active layer OECTs, a mi RNA24 biosensor based on electromechanical chemical transistors is constructed with a gold electrode modified with HS-DNA (complementary to MI RNA24) as a gate electrode. Under optimal detection conditions, the detection limit of the sensor to MI RNA24 is 20 pM; the linear range is 1 nM-200 nM; sensitivity is good and selectivity is good. Well, the response of miRNA29 and mismatch miRNA24 to the sensor is small. (2) the Au-S bond is fixed on the surface of the gold electrode and HS-DNA is fixed on the surface of the gold electrode, and as a OECTs gate electrode, a electromechanical chemical transistor miRNA24 biosensor based on the amplification of the potassium ferrocyanide probe signal is constructed, and its performance is studied in detail. The detection limit of the sensor to miRNA24 is 30 fM, the linear range is 10 pM-10 nM, and the sensitivity is 0.05989 decade-1. (3) based on the OECTs device of the carbon pulp electrode and Au/Ti electrode. The transmission performance, the output performance and the stability of the OECTs device are studied in detail. In addition, in order to improve the sensitivity of the sensor, I We electrodeposited nanoscale gold on the gate electrode and constructed a miRNA24 biosensor based on gold nanoparticles. The feasibility of the biosensor to detect mi RNA24 was preliminarily studied.

【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TN32;O657.1
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本文编号：1847654