浅谈高质量透射电镜照片的拍摄

发布时间：2018-06-10 20:38

本文选题：透射电子显微镜 + 样品制备　；参考：《实验室研究与探索》2016年07期

【摘要】：透射电子显微镜(Transmission Electron Microscope,TEM)具有很高的分辨率和放大倍数高,广泛应用于实验教学和科学研究各个领域。透射电子显微镜结合不同的附件(X射线能谱分析(EDS)、电子能量损失谱(EELS)),可以同时提供形貌、成分、结构信息,它可以揭示物质内部的微细观结构,是人们了解、认识事物微观结构不可缺少的工具。观察透射电镜的最终目的是得到清晰的照片,而样品的制备是关键。透射电镜样品的制备质量,直接影响到样品的观察效果与分析结果。本文以JEM-2100型透射电子显微镜为例,从样品制备方法的选择、载网与支持膜的选用、样品防污染措施及电镜操作等几方面入手,详细地介绍高质量的透射电镜照片的拍摄经验,为透射电镜相关工作人员提供技术参考。
[Abstract]:Transmission Electron MicroscopeTem (TEM) is widely used in experimental teaching and scientific research because of its high resolution and high magnification. The transmission electron microscope (TEM) combined with different accessory X-ray energy spectra (EDS), the electron energy loss spectrum (EELS), can provide the information of morphology, composition and structure at the same time. It can reveal the microstructures inside the matter and is understood by people. An indispensable tool for understanding the microstructure of things. The final aim of TEM observation is to obtain clear photographs, and the preparation of samples is the key. The preparation quality of TEM samples has a direct effect on the observation and analysis results. Taking JEM-2100 transmission electron microscope as an example, this paper introduces the experience of taking high-quality transmission electron microscope photos in detail from the aspects of sample preparation method, selection of carrier net and supporting membrane, sample anti-pollution measures and electron microscope operation. To provide technical reference for transmission electron microscope related staff.
【作者单位】：华南师范大学实验中心;广东工业大学期刊中心;
【分类号】：TN16

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