基于微流控芯片的量子点生物标记研究

发布时间：2019-05-27 07:49

【摘要】：微流控芯片是将生物化学的基本功能集合到一个较小的平台,展现出便携化、成本低、环境污染小、通道设计灵活、集成度高及多功能化的优势,为化学生物的研究提供了更为便捷的方式。本论文基于微流控芯片进行了量子点生物标记的实验研究,研究了微流控芯片的制作及量子点生物标记的应用。首先利用COMSOL多物理场软件对微通道的不同通道结构尺寸下的速度场进行模拟分析,得出适用于细胞培养的微通道尺寸,然后制作出微流控芯片并对其进行表面修饰,改变基底材料的表面疏水性使其适合贴壁细胞培养,实现其生物化功能。最后结合修饰有叶酸的三元量子点(Cu In S2),因其具有较低的毒性,较好的生物兼容性,光谱不宜重叠,荧光信号便于检测的特点,实现芯片上量子点生物标记。将微流控芯片与三元量子点结合的方式,达到了样品消耗少,能够实现定量分析,提高量子点标记的检测灵敏度,可见微流控芯片在量子点荧光标记上的应用是有广泛前景的。
[Abstract]:Microfluidic chip is a kind of microfluidic chip, which integrates the basic functions of biochemistry into a small platform, showing the advantages of portability, low cost, low environmental pollution, flexible channel design, high integration and multifunction. It provides a more convenient way for the study of chemistry and biology. In this paper, the experimental study of quantum dot biomarker based on microfluidic chip is carried out, and the fabrication of microfluidic chip and the application of quantum dot biomarker are studied. Firstly, the velocity field under different channel structure dimensions of microchannel is simulated and analyzed by COMSOL multi-physical field software, and the microchannel size suitable for cell culture is obtained, and then the microfluidic chip is fabricated and modified on the surface. Changing the surface hydrophobicity of the substrate material makes it suitable for adherent cell culture and realizes its biological function. Finally, the ternary quantum dots (Cu In S2) modified with folic acid are combined with the characteristics of low toxicity, good biocompatibility, unoverlapping spectra and easy detection of fluorescence signals, so that the biomarker of QDs on the chip is realized. By combining microfluidic chip with ternary quantum dot, the sample consumption is less, the quantitative analysis can be realized, and the detection sensitivity of quantum dot labeling can be improved. It can be seen that the application of microfluidic chip in quantum dot fluorescence labeling has a wide prospect.
【学位授予单位】：长春理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q6-3;TN492

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