基于发光细菌法和鱼类暴露实验评价6种化学消油剂的遗传毒性

发布时间：2018-05-10 08:04

本文选题：化学消油剂 + 遗传毒性　；参考：《应用与环境生物学报》2017年01期

【摘要】：为评价海洋溢油应急处置中所使用的消油剂的环境安全性,分别以发光细菌Acinetobacter sp.Rec A和海水青溕(Oryzias melastigma)幼鱼为受试生物,对6种化学消油剂的遗传毒性进行检测.结果显示,采用发光细菌法仅检测到HLD-501(常规型)在浓度较高(500 mg/L和1 000 mg/L)时表现出一定水平的遗传毒性,其等效应的丝裂霉素C(MMC)浓度分别为0.039 mg/L和0.032 mg/L.在彗星实验中,6种消油剂对海水青溕幼鱼细胞的DNA损伤率从高到低依次为RS-II(浓缩型)、RS-I(浓缩型)、HLD-501(常规型)、富肯-2(常规型)、RS-I(常规型)和微普(常规型),然而HLD-501(常规型)处理的3级损伤率最高,其遗传毒性最大.综上,采用两种不同营养级的受试生物对6种化学消油剂遗传毒性检测结果的相关度较好;本研究可为消油剂用于处理实际海洋溢油污染的环境安全性评价提供参考.
[Abstract]:In order to evaluate the environmental safety of oil suppressants used in marine oil spill emergency disposal, the genotoxicity of six chemical oil suppressants was detected by using Acinetobacter sp.Rec A and Oryzias melastigma as the tested organisms. The results showed that HLD-501 (conventional type) showed a certain level of genotoxicity at a higher concentration of 500 mg/L and 1 000 mg / L, and its isoeffect mitomycin C MMC concentration was 0.039 mg/L and 0.032 mg / L, respectively. In comet assay, the DNA damage rate of six kinds of oil suppressants to young fish cells from high to low is RS-IIII (condensed HL-D-501), Fucen-2 (conventional), HLD-501 (conventional) and microbutene (conventional), but HLD-501 (conventional). The third grade damage rate was the highest. Its genetic toxicity is the greatest. In conclusion, the genetic toxicity test results of six chemical oil suppressants were well correlated with two kinds of tested organisms with different nutrient levels, and this study could provide a reference for environmental safety evaluation of oil elimination agents used in the treatment of actual marine oil spill pollution.
【作者单位】：青岛科技大学化工学院;国家海洋局第一海洋研究所海洋生态研究中心;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室;
【基金】：中央级公益性科研院所基本科研业务费专项资金(2014G12,2015T05) 青岛市黄岛区海洋科技专项(2014-4-20)资助~~
【分类号】：X55;X171.5

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