裂褶菌MCCC 3A00233对菲、荧蒽降解作用的研究
发布时间:2018-05-19 02:07
本文选题:微生物学 + 裂褶菌 ; 参考:《中国科技论文》2016年18期
【摘要】:采用平板和液体培养技术,研究了1株海洋源裂褶菌(Schizophyllum commune MCCC 3A00233)降解多环芳烃(polycyclic aromatic hydrocarbons,PAHs)类化合物菲、荧蒽的效率及机理。结果表明:裂褶菌MCCC 3A00233对菲、荧蒽具有较高的耐受性,在质量浓度高达200mg/L的平板上,菌丝生长的抑制率≤25%;当菲、荧蒽的质量浓度为50mg/L时,菌株生长最快,培养10d对菲、荧蒽的降解率≥80%,降解产物对植物、微生物的毒性大大减小。另外,该菌对菲、荧蒽的降解作用主要是通过产生锰过氧化物酶(manganese peroxidase,MnP),其次为木质素过氧化物酶(lignin peroxidase,LiP)和漆酶(Laccase)而起作用的,其中Lip和Laccase在该菌降解菲、荧蒽的起始阶段发挥重要作用。这些结果表明,裂褶菌MCCC 3A00233是1株高效降解菲、荧蒽的白腐真菌,可用于海水中PAHs类污染物的降解。
[Abstract]:The efficiency and mechanism of degradation of phenanthrene (phenanthrene) and fluoranthene (fluoranthene) by a strain of Schizophyllum commune MCCC 3A00233) were studied by plate and liquid culture techniques. The results showed that MCCC 3A00233 had higher tolerance to phenanthrene and fluoranthene, and the inhibition rate of hyphal growth was 鈮,
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