流动注射Ferene分光光度法测定海水中溶解态铁的方法优化

发布时间：2018-06-10 18:00

本文选题：溶解态铁 + Ferene　；参考：《海洋科学》2016年05期

【摘要】：热液流体中溶解态铁是海水原位测量的重要参数之一。本研究采用Ferene分光光度法,搭建流动注射分析系统,优化进样条件、显色条件,实现了热液流体中溶解态铁的在线测定。结果表明,测定Fe(II)时,Ferene、缓冲液浓度分别为8×10 3、0.4 mol/L,Ferene、样品流速分别为0.8、0.6 m L/min,显色盘管长度为40 cm时,方法的灵敏度、检测限最佳;测定Fe(III)时,Ferene、缓冲液、抗坏血酸浓度分别为1×10 2、0.5、0.01 mol/L,Ferene/抗坏血酸、样品流速均为1.0 m L/min,还原、显色盘管长度均为40 cm时,方法的灵敏度、检测限最佳。最佳实验条件下,Fe(II)、Fe(III)在0.2~10μmol/L和0.5~16μmol/L范围内,工作曲线回归方程分别为A=0.0834 C+0.0564(μmol/L,n=8,R2=0.997)和A=0.0478C+0.0423(μmol/L,n=8,R2=0.997)。Fe(II)、Fe(III)检测限分别为24、39 nmol/L,相对标准偏差分别为0.8%、1.2%(n=10),加标回收率为97.9%~103.0%。共存离子实验表明,流体中的Na+、Mn2+、Cu2+、Cu+不会对测量造成干扰。
[Abstract]:Dissolved iron in hydrothermal fluid is one of the important parameters in situ measurement of seawater. In this study, a flow injection analysis system was set up by using Ferene spectrophotometry. The on-line determination of dissolved iron in hydrothermal fluid was realized by optimizing the conditions of injection and coloration. The results showed that the sensitivity and the detection limit of the method were the best when the concentration of Ferenewas 8 脳 10 ~ (3) O ~ (3) mol 路L ~ (-1) 路L ~ (-1), the flow rate of the sample was 0.80.6mL / min, the length of the color coil was 40 cm, the sensitivity and the detection limit of the method were the best when the Fe ~ (2 +) was determined, and the buffer solution was used in the determination of Fe ~ (2 +). When the concentration of ascorbic acid was 1 脳 10 ~ (2) ~ (2) C ~ (0.5) mol 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路min, the sensitivity and detection limit of the method were the best when the velocity of the sample was 1.0 mL / min, the length of the reduction and the length of the chromogenic coil were 40 cm. Under the optimum experimental conditions, the working curve regression equations were A0.0834 C 0.0564 (渭 mol / L) and A0.0478C 0.0423 (渭 mol / L Lnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntl) 0.997nmol / L (渭 mol / L) 0.997nmol / L and 0.516 渭 mol / L = 0.516 渭 mol / L, respectively, and the relative standard deviations were 0.892 nmol / L and 97.992% ~ 103.0, respectively. The results showed that the relative standard deviation (RSD) was 0.839 nmol / L and the relative standard deviation (RSD) was 0. 839 nmol / L and 97. 990%, respectively, and the recovery was 97. 970 nm / L and the recoveries were 97. 990% and 103. 0%, respectively. The experimental results of coexisting ions show that the Na ~ (2 +) mn _ (2) Cu _ (2) O _ (2) Cu in the fluid will not interfere with the measurement.
【作者单位】：国家深海基地管理中心;
【基金】：国家自然科学基金项目(41306103) 国家重点基础研究973项目课题(2015CB755901) 海洋公益性行业专项经费资助项目(201405036)~~
【分类号】：P714

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