TBBPA对海洋微藻毒性效应的研究

发布时间：2018-06-24 15:40

本文选题：TBBPA + 海洋微藻　；参考：《中国海洋大学》2014年硕士论文

【摘要】：四溴双酚A(TBBPA)是目前世界上产量和用量最大的溴系阻燃剂，相关研究表明其对水生生物具有较强的急性毒性。海洋微藻作为海洋态系统中的初级生产者，在维持水生态的稳定与平衡中起着重要作用。基于目前有关TBBPA对海洋微藻的毒理学研究还相当少见，本文开展了TBBPA对海洋微藻毒性效应的相关研究，为全面评价TBBPA对海洋环境的影响作出初步探索。主要内容分为以下三个部分，（1）TBBPA对七种海洋微藻生长的影响。（2）TBBPA与B[a]P对塔胞藻和湛江等鞭金藻联合毒性效应的研究。（3）TBBPA与Cu2+对塔胞藻和湛江等鞭金藻毒性效应的研究。主要结果如下： 1．TBBPA对七种海洋微藻生长的影响以7种海洋微藻（微拟球藻、塔胞藻、牟氏角毛藻、湛江等鞭金藻、等鞭金藻塔溪）为研究对象，通过分析TBBPA处理下海洋微藻相对生长率、抑制率的变化，同时用概率单位—浓度对数法求得其96h EC50，来研究TBBPA对7种海洋微藻生长的影响。结果显示：TBBPA对微拟球藻、塔胞藻、牟氏角毛藻、湛江等鞭金藻、等鞭金藻塔溪堤品系的96h EC50分别为2.64mg/L、6.71mg/L、2.59mg/L、4.35mg/L、4.23mg/L，三角褐指藻在浓度为9.83mg/L的TBBPA的作用下在96h时抑制率为22.8%，亚心形扁藻在浓度14.6mg/L的TBBPA作用下在96h的抑制率仅为17.1%。牟氏角毛藻、微拟球藻为对TBBPA最为敏感的海洋微藻。 2. TBBPA与B[a]P对塔胞藻和湛江等鞭金藻联合毒性效应的研究本文依据预实验设计了合适的污染物配比浓度，先研究TBBPA、B[a]P及TBBPA+B[a]P对海洋微藻生长的影响，分别求得三种情况下微藻的96h EC50，然后采用Marking指数相加法来评价TBBPA与B[a]P对塔胞藻和湛江等鞭金藻联合毒性效应。结果表明：（1）TBBPA对湛江等鞭金藻的毒性作用属高毒，B[a]P对湛江等鞭金藻的作用属于极高毒。TBBPA与B[a]P在500:3的混合比例下，TBBPA与B[a]P的联合毒性指数AI值为-1.23，小于0，为拮抗作用。（2）TBBPA对塔胞藻的毒性作用属高毒，B[a]P对塔胞藻的作用属于高毒。在4:1的混合比例下，联合毒性指数AI值为-2.03，小于0，故为拮抗作用。 3. TBBPA与Cu2+对塔胞藻和湛江等鞭金藻联合毒性效应的研究本文依据预实验设计了合适的污染物配比浓度，，先研究TBBPA、Cu2+及TBBPA+Cu2+对海洋微藻生长的影响，分别求得三种情况下微藻的96h EC50，然后采用Marking指数相加法来评价TBBPA与Cu2+对塔胞藻和湛江等鞭金藻联合毒性效应。结果表明：（1）Cu2+对湛江等鞭金藻的作用属于高毒。在5：1的混合比例下，TBBPA与Cu2+联合毒性指数AI值为-1.23，小于0，为拮抗作用。（2）Cu2+对塔胞藻的作用属于中毒。在5:2的混合比例下，TBBPA与Cu2+联合毒性指数AI值为-1.38，小于0，为拮抗作用。
[Abstract]:Tetrabromobisphenol A (TBBPA) is the largest bromine flame retardant in the world at present. Related studies have shown that TBBPA has a strong acute toxicity to aquatic organisms. Marine microalgae, as primary producers in marine systems, play an important role in maintaining the stability and balance of water ecology. Based on the relatively rare toxicological studies of TBBPA on marine microalgae, this paper has carried out related studies on the toxic effects of TBBPA on marine microalgae, in order to evaluate the effects of TBBPA on marine environment. The main contents are as follows: (1) the effect of TBBPA on the growth of seven marine microalgae; (2) the study on the toxic effects of TBBPA and B [a] P on TBBPA and Zhanjiang isopodium. (3) the toxic effects of TBBPA and Cu2 on TBBPA and Zhanjiang isopodium. The main results are as follows: 1. The effects of TBBPA on the growth of seven marine microalgae were studied. The effects of TBBPA on the growth of seven marine microalgae were studied by analyzing the changes of the relative growth rate and inhibition rate of marine microalgae under TBBPA treatment, and the 96 h EC50 was obtained by using probabilistic unit concentration logarithm method to study the effect of TBBPA on the growth of seven marine microalgae. The results showed that the effects of TBBPA on Chlorella microphylla, Phaeocystis monocystis, Cham's Corydophyta, Zhanjiang isopodium were studied. The 96 h EC50 of TBBPA was 2.64 mg / L 6.71 mg / L 2.59 mg / L 4.35 mg / L 4.23 mg / L, the inhibition rate of Phaeopsis triangularis was 22.8 at 96 h under the action of 9.83 mg / L TBBPA, and the inhibition rate of TBBPA at 14.6 mg / L was 17.1mg / L at 96 h. Chlorella mongolica is the most sensitive marine microalgae to TBBPA. Effects of TBBPA and B [a] P on the combined toxicity of TBBPA and B [a] P on the growth of marine microalgae, the suitable concentration of pollutants was designed based on the pre-experiment. The effects of TBBPA B [a] P and TBBPA B [a] P on the growth of marine microalgae were studied firstly, and the effects of TBBPA B [a] P and TBBPA B [a] P on the growth of marine microalgae were studied. The 96 h EC50 of microalgae was obtained under three conditions, and the combined toxicity of TBBPA and B [a] P to Phaeophyta and Zhanjiang isopodium was evaluated by Marking index addition method. The results showed that: (1) the toxic effect of TBBPA on Isochrysis zhanjiang belongs to the highly toxic B [a] P, which belongs to the extremely high toxicity. TBBPA and B [a] P in the mixture ratio of 500: 3 and B [a] P, the joint toxicity index AI of TBBPA and B [a] P is -1.23, less than 0, which is antagonistic effect. (2) the toxic effect of TBBPA on TBBPA belongs to high toxic B [a] P. Under the mixture ratio of 4:1, the AI value of joint toxicity index was -2.03, less than 0, so it was antagonistic. Effects of TBBPA and Cu _ 2 on the combined toxicity of TBBPA and Cu _ 2 on the growth of marine microalgae the suitable concentration of pollutants was designed based on the pre-experiment. The effects of TBBPA Cu2 and TBBPA Cu2 on the growth of marine microalgae were studied. The 96 h EC50 of microalgae was obtained under three conditions, and the combined toxicity of TBBPA and Cu2 to Phaeophyta and Zhanjiang isopodium was evaluated by Marking index addition method. The results showed that: (1) the effect of Cu _ 2 on isopodium zhanjiang belongs to high toxicity. The combined toxicity index (AI) of TBBPA and Cu2 was -1.23 at 5:1, which was antagonistic. (2) the effect of Cu2 on TBBPA and Cu2 was toxic. The combined toxicity index (AI) of TBBPA and Cu2 was -1.38, less than 0 at 5:2, which was antagonistic.
【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：X171.5;X55

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