尿素对中肋骨条藻与米氏凯伦藻生长的影响

发布时间：2018-10-26 10:49

【摘要】：采用实验室一次性培养,研究了尿素对我国东海赤潮优势藻中肋骨条藻(Skeletonema costatum)和米氏凯伦藻(Karenia mikimotoi)生长的影响。结果表明,中肋骨条藻和米氏凯伦藻均能在不同比例尿素的条件下较好地生长。随着培养液中尿素比例的增大,中肋骨条藻细胞生长速率(0.91—0.82/d)逐渐减小,平台期最大生物量(2.0×10~5—1.2×10~5个/m L)也逐渐减小,而米氏凯伦藻细胞的生长速率(0.36—0.51/d)逐渐增大,最大生物量基本不变(约1.1×10~4个/m L)。在平台期中肋骨条藻培养液中氮盐浓度最低下降到2.5μmol/L左右维持不变,而米氏凯伦藻氮盐浓度最低下降到1.0μmol/L左右。在指数生长期,随着细胞的生长溶解有机氮(DON,Dissolved Organic Nitrogen)含量迅速增加,中肋骨条藻介质中DON的浓度达到最大值(5—6μmol/L),然后浓度基本不变。米氏凯伦藻介质中DON在指数生长阶段达到最大值(2—3μmol/L)后开始下降。中肋骨条藻单细胞颗粒氮的含量(约为10~(-6)μmol,平台期约为10~(-7)μmol)要远远小于米氏凯伦藻(指数期约为10~(-4)μmol,平台期约为10~(-6)μmol)。研究表明,两种藻对尿素的吸收利用存在明显差异,在较低的溶解无机氮和较高的溶解有机氮环境中,甲藻有更好的适应性,该研究对于解释我国长江口春季硅藻和甲藻赤潮的演替有借鉴的意义。
[Abstract]:The effects of urea on the growth of (Skeletonema costatum) and (Karenia mikimotoi) in red tide dominant algae in the East China Sea were studied in laboratory. The results showed that both of them could grow well under different proportion of urea. With the increase of urea ratio in culture medium, the growth rate (0.91-0.82 / d) and the maximum biomass (2.0 脳 10 ~ (-5) -1.2 脳 10 ~ (5) / m L) decreased gradually. However, the growth rate (0.36-0.51 / d) of Kellenia mirolimi cells gradually increased, and the maximum biomass (about 1.1 脳 10 ~ 4 / m L).) was almost unchanged (1. 1 脳 10 ~ 4 / m L). The lowest concentration of nitrogen salt in the medium of platformis costatum decreased to 2. 5 渭 mol/L or so, while the lowest concentration of nitrogen salt in algal Carallina mikeli decreased to about 1. 0 渭 mol/L. During exponential growth period, the concentration of DON reached the maximum (5-6 渭 mol/L) in medium with the rapid increase of the content of dissolved organic nitrogen (DON,Dissolved Organic Nitrogen), and the concentration remained unchanged. The DON in the medium of Karen's algae began to decrease after reaching the maximum value (2-3 渭 mol/L) during exponential growth. The content of particulate nitrogen in single cell of S.costatum (about 10 ~ (-6) 渭 mol, platform is 10 ~ (-7) 渭 mol) is much smaller than that of Karen's algae (the exponential period is about 10 ~ (-4) 渭 mol, platform stage is about 10 ~ (-6) 渭 mol). The results showed that there were obvious differences in urea absorption and utilization between the two algae. In the environment of lower dissolved inorganic nitrogen and higher dissolved organic nitrogen, the algae had better adaptability. This study can be used for reference to explain the succession of diatoms and pyrophyta red tide in spring in the Yangtze Estuary of China.
【作者单位】：中国海洋大学化学化工学院;中国海洋大学海洋化学理论与工程技术教育部重点实验室;国家海洋局海洋减灾中心;
【基金】：国家自然科学基金资助项目(41376106)
【分类号】：X55

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