海洋酸化对日本虎斑猛水蚤抗氧化性指标和繁殖发育的影响

发布时间：2018-11-25 06:54

【摘要】：本文选取海洋桡足类日本虎斑猛水蚤(Tigriopus japonicus)为模式生物,采用生化测定和实验生态学方法,研究不同酸化胁迫对桡足类体内抗氧化性指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)和脂质过氧化(LPO)]、幼体发育时间、世代发育时间和产后代数的影响。结果表明:海水酸化可显著诱导桡足类的抗氧化性酶(SOD和CAT)的活性,而GST活性不受酸化影响,同时酸化可显著提高桡足类的LPO水平,可见该桡足类可通过SOD和CAT活性的提高来抵抗酸化胁迫引起的氧化胁迫;另外,酸化显著缩短桡足类的幼体发育时间和世代发育时间,并显著抑制其产后代数,提示酸化可以影响海洋桡足类的种群补充和动态;酸化引起该桡足类氧化胁迫的出现,继而对细胞生物大分子(如蛋白质、核酸等)造成氧化损伤,最终影响到该动物的个体水平和种群动态(如缩短发育时间和抑制繁殖力)。
[Abstract]:In this paper, the marine copepod (Tigriopus japonicus) was selected as the model organism, and the antioxidant index (SOD),) of copepods under different acidification stress was studied by biochemical and experimental ecological methods. Effects of catalase (CAT), glutathione S-transferase (GST) and lipid peroxidation (LPO)], larval development time, generation development time and postpartum algebra. The results showed that seawater acidification could significantly induce the activities of antioxidant enzymes (SOD and CAT) in copepods, while the activity of GST was not affected by the acidification, and the level of LPO in copepods was significantly increased by acidification. It can be seen that the copepod can resist oxidative stress induced by acidification by increasing the activity of SOD and CAT. In addition, acidification significantly shortened the larval development time and generation development time of copepods, and significantly inhibited their postpartum algebra, suggesting that acidification could affect the population replenishment and dynamics of marine copepods. Acidification caused oxidative stress of copepods, which resulted in oxidative damage to cellular macromolecules (such as protein, nucleic acid, etc.), and ultimately affected the individual level and population dynamics (such as shortening development time and inhibiting fecundity).
【作者单位】：国家海洋局厦门海洋环境监测中心站;
【基金】：国家海洋局东海分局青年科技基金资助项目(201321) 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室资助课题(MATHAB201409)资助
【分类号】：S917.4;P734

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