栉孔扇贝在镉污染胁迫下消化盲囊组织的转录组分析

发布时间：2019-01-23 15:15

【摘要】：镉(Cd)作为生物非必需、毒性极强的蓄积性重金属,易通过食物链进入人体,严重威胁人类健康。扇贝相对于其他贝类具有特异性蓄积镉的能力,成为水产品安全领域关注的焦点。为了阐释扇贝高蓄积镉的分子机制,本研究以镉胁迫的栉孔扇贝(Chlamys farreri)消化盲囊组织为研究对象,通过转录组测序技术进行基因转录水平分析。通过比较转录组拼接获得105071个unigene和3800个差异基因,对所得unigene进行功能注释,发现这些基因集中在蛋白绑定、细胞黏附、免疫应答、细胞凋亡和能量代谢等方面。对这些蛋白的分子功能进行注释,发现该类蛋白主要属结合蛋白(40.45%)、催化活性蛋白(34.27%)和转运蛋白(5.62%)。这些功能基因和预测通路为理解扇贝体内解毒和免疫系统奠定了基础。获得的转录组数据为深入研究双壳贝类应对海洋污染物的分子机制提供了丰富的基因资源。
[Abstract]:Cadmium (Cd) (CD (Cd), as a non-essential and highly toxic accumulative heavy metal, is easy to enter the human body through the food chain and is a serious threat to human health. Scallop has the ability to accumulate cadmium specifically compared with other shellfish and has become the focus in aquatic product safety field. In order to elucidate the molecular mechanism of cadmium accumulation in scallop chlamys farreri (Chlamys farreri) digesting blind capsule tissue under cadmium stress was used to analyze the gene transcription level by transcriptional sequencing technique. 105071 unigene and 3800 differentially expressed genes were obtained by comparing transcriptome splicing. The functional annotation of the unigene showed that these genes focused on protein binding, cell adhesion, immune response, apoptosis and energy metabolism. The molecular function of these proteins was elucidated. It was found that these proteins mainly belong to binding protein (40.45%), catalytic active protein (34.27%) and transporter protein (5.62%). These functional genes and predictive pathways lay the foundation for understanding the detoxification and immune system in scallop. The obtained transcriptome data provide abundant genetic resources for further study on the molecular mechanism of bivalve in response to marine pollutants.
【作者单位】：农业部水产品质量安全检测与评价重点实验室中国水产科学研究院黄海水产研究所;农业部水产品质量安全风险评估实验室(青岛);
【基金】：国家自然科学基金项目(41506134)
【分类号】：S917.4;X55

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