不同海洋微藻对石油污染的响应差异

发布时间：2019-07-17 08:06

【摘要】：微藻对油污染物的不同响应,势必造成其群落种类组成和结构的变化,进一步影响海洋生态系统的变迁。由于油污染对于浮游植物的影响具有污染源及生物响应的多样性、多维度性和复杂性,产生影响的时效长但可显示度低等特点,造成该研究领域的极大限制。作者以石油标准品20-3和3种石油化学品(双酚、对苯二甲酸、对二甲苯)作为油污染源,利用多孔板高通量检测方法及大数据处理分析软件Simca-P,对中国沿海常见单种微型藻类的种群消长受油污染影响进行了研究。以石油标准品20-3为油污染源,分析了31株微藻增殖对不同油标浓度的不同响应;利用Simca-P软件对3种不同浓度的石油化学品对4种典型微藻(柔弱角刺藻(Chaetoceros debilis)、球等鞭金藻(Isochrysis galbana)、东海原甲藻(Prorocentrum donghaiense)、剧毒卡罗藻(Karlodinum veneficum))增殖的影响进行了比较分析。研究结果显示:不同种类微藻因油污种类及其浓度不同,其响应存在显著差异。利用多孔板以及微藻的吸光特征,可以高通量快速筛查不同微藻对不同油污及其不同浓度的响应差异,并利用计算机软件对大数据的分析能力,挖掘和比较不同微藻与不同油污之间的相互关系,从而就油污染对微藻影响这种多维度复杂关系进行研究。
[Abstract]:The different response of microalgae to oil pollutants is bound to cause the change of community species composition and structure, and further affect the changes of marine ecosystem. Because of the diversity of pollution sources and biological response, multi-dimension and complexity, the effect of oil pollution on zooplankton has the characteristics of long time limit but low display degree, which leads to great limitation in this research field. The results show that oil pollution has the characteristics of diversity of pollution sources and biological response, multi-dimension and complexity, and the long time limit of the influence. Using petroleum standard 20o3 and three petroleum chemicals (bisphenol, terephthalic acid, p-xylene) as oil pollution sources, the influence of oil pollution on the population growth and decline of single microalgae in coastal China was studied by using porous plate high throughput detection method and big data treatment and analysis software Simca-P,. Using petroleum standard 20o3 as oil pollution source, the different responses of 31 strains of microalgae proliferation to different oil standard concentrations were analyzed, and the effects of three different concentrations of petroleum chemicals on the proliferation of four typical microalgae, such as (Chaetoceros debilis), ball and so on, were compared and analyzed in this paper. The effects of three different concentrations of petroleum chemicals on the proliferation of four typical microalgae, such as (Chaetoceros debilis), ball and so on, were compared and analyzed. The results showed that the responses of different species of microalgae were significantly different due to the different types and concentrations of oil pollution. By using the absorption characteristics of porous plates and microalgae, the responses of different microalgae to different oil pollution and their concentrations can be screened quickly and high throughput, and the analysis ability of big data by computer software is used to excavate and compare the relationship between different microalgae and different oil pollution, so as to study the multi-dimensional complex relationship of the influence of oil pollution on microalgae.
【作者单位】：宁波大学海洋学院;
【基金】：中国国家科技部星火计划项目(2015GA701001) 浙江省自然科学基金资助项目(LY12D06001) 国家公益性行业(海洋)科研专项经费项目(201105009) 海洋可再生能源专项资金项目(GHME2001SW02) 国家“万人计划”(财教[2014]412号)~~
【分类号】：X55

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