共基质型微生物燃料降解甲基橙的实验研究

发布时间：2018-02-23 20:23

  本文关键词： 微生物燃料电池 共基质 甲基橙 硝酸盐 产电　出处：《合肥工业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本文通过构建共基质型微生物燃料电池(microbial fuel cell,MFC)在阳极室降解甲基橙,研究甲基橙的降解途径和中间产物,重点关注运行过程中阳极的关键条件与参数对甲基橙脱色和产电特性的影响。在此基础上,进一步将非生物阴极更换为生物阴极,分析其用于同步降解硝酸盐和甲基橙的可行性。主要结论如下:本研究构建的MFC,以甲基橙和葡萄糖的混合基质为燃料,最大输出电压为0.51 V,甲基橙脱色率能高达96.38%。对脱色液进行液相色谱-质谱(liquid chromatograph mass spectrometer,LC-MS)检测,得出主要中间产物为对氨基苯磺酸和氮氮二甲基对苯二胺。本研究探讨了阳极的关键参数对MFC的甲基橙脱色以及产电性能的影响。结果显示低浓度(100-300 mg/L)的甲基橙对MFC的电压输出影响不大,微生物对低浓度甲基橙存在一定程度的耐受性;当甲基橙初始浓度为300 mg/L,得出最佳共基质浓度为500 mg/L,用以满足甲基橙的脱色以及微生物的生长需求;pH可以影响微生物的活性从而会改变甲基橙的脱色性能,当阳极液的pH为6.80时,微生物的代谢作用最强,故MFC的脱色效果最佳,脱色率在12 h已经超过了90.00%,化学需氧量(chemical oxygen demand,COD)去除率达到57.25%;外接电阻较低会加快电子的传递,同时甲基橙脱色率和COD去除率能达到较高的水平;高温对MFC系统的脱色和产电性能产生明显的影响,当运行温度为35℃时,MFC的产电和脱色效果最好;MFC脱色甲基橙主要是依靠阳极表面附着的微生物的新陈代谢,但是一定量的悬浮污泥不仅能增加输出电压,还能提高甲基橙的脱色率和COD的去除率。当初期构建的共基质型MFC的各性能处于稳定状态后,改为还原硝酸盐的共基质型生物阴极MFC,并得到以下结果:当阴极硝酸盐浓度为50 mg/L时,该生物阴极MFC的平均的反硝化速率能达到2.015 mg/(L?d),最高输出电压为0.3 V,反应48 h甲基橙脱色率能达到72.36%,COD去除率为43.24%。本研究构建的共基质型MFC可以有效的降解甲基橙,在此基础上构建的共基质型生物阴极MFC,可以在阴阳两室同步降解污染物并达到产电目的,具有无污染、应用范围广、电池效率高等特点,为偶氮染料废水的处理提供了一个全新的思路。
[Abstract]:In this paper, the degradation pathway and intermediate product of methyl orange in anodic chamber were studied by constructing co-substrate microbial fuel cell. The influence of the key conditions and parameters of the anode on the decolorization and electrical characteristics of methyl orange is focused on. On this basis, the abiotic cathode is further replaced by a biological cathode. The feasibility of simultaneous degradation of nitrate and methyl orange was analyzed. The main conclusions were as follows: the MFC was constructed using the mixed matrix of methyl orange and glucose as fuel. The maximum output voltage is 0. 51 V, and the decolorization rate of methyl orange can reach 96.38. The liquid chromatography-mass spectrometry (LC-MS) is used to detect the decolorization solution by liquid chromatography-mass spectrometry (LC-MS). The main intermediate products were p-aminobenzenesulfonic acid and nitrogen-dimethyl p-phenylenediamine. The effect of the key parameters of anode on decolorization and electrical properties of methyl orange of MFC was investigated. The results showed that the concentration of methyl orange was 100-300 mg / L of low concentration. It has little effect on the voltage output of MFC. The microbes had some tolerance to low concentration methyl orange. When the initial concentration of methyl orange is 300 mg / L, the optimum co-substrate concentration is 500 mg / L, which can be used to meet the decolorization of methyl orange and the growth demand of microorganisms. When the pH value of anodic solution was 6.80, the metabolism of microorganism was the strongest, so the decolorization rate of MFC was the best, the decolorization rate exceeded 90.00. the removal rate of chemical oxygen and COD reached 57.25%, and the low external resistance accelerated the electron transfer. At the same time, the decolorization rate of methyl orange and the removal rate of COD can reach a higher level, and the high temperature has a significant effect on the decolorization and electrical properties of MFC system. When the operating temperature is 35 鈩，

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