手性荧光复合微球的制备及表征

发布时间：2018-03-20 05:21

本文选题：二氧化硅　切入点：螺旋聚合物　出处：《北京化工大学》2015年硕士论文　论文类型：学位论文

【摘要】：本论文利用丙炔胺分别和丙烯酸及Boc-L-丙氨酸反应合成了两种取代乙炔类单体,即手性Boc-L-丙氨酸丙炔酰胺单体1和非手性丙烯酸丙炔酰胺单体2。两种单体在催化剂(nbd)Rh+[η6-C6H5B-(C6H5)3]作用下进行均(共)聚合得到产率57%-78%,数均分子量Mn=1.8×104-2.0 x 104的三种聚合物。圆二色光谱(CD)、紫外-可见吸收光谱(UV-vis)及旋光度([α]D)测试表明手性单体的均聚物及共聚物均具有很强的光学活性,并且聚合物主链具有单手性螺旋构象。本论文研究了温度、侧基及非手性单体对聚合物二级结构的影响。共聚物在三氟乙酸/二氯甲烷混合溶液作用下脱掉侧链上的叔丁氧羰基(Boc)基团得到带有氨基的螺旋聚合物,这种带有特殊官能团的螺旋聚合物将用于下一步的接枝反应。本论文使用无机二氧化硅纳米粒子为载体制备手性荧光复合微球。首先用3-氨丙基三甲氧基硅烷及丁二酸酐对二氧化硅纳米粒子进行化学改性,使二氧化硅纳米粒子表面带有羧基,并使用滴定方法研究了二氧化硅纳米粒子表面的羧基含量。其次,在脱水剂及催化剂作用下,将带有氨基的螺旋聚合物通过共价键作用接枝到二氧化硅纳米粒子表面。通过热失重曲线上研究了二氧化硅纳米粒子表面螺旋聚合物的接枝率。最后通过蒸馏沉淀聚合方法将荧光性能的聚合物包覆在手性复合微球表面,制备了一种新型的手性荧光复合微球。本论文利用扫描电子显微镜及透射电子显微镜观察了手性荧光复合微球的表面形貌及粒径。紫外-可见吸收光谱及荧光光谱表明手性荧光复合微球具有一定的荧光性能。这种手性荧光复合微球将在生物标记及示踪学领域发挥重要作用。
[Abstract]:In this paper, two substituted acetylene monomers were synthesized by the reaction of propargmine with acrylic acid and Boc-L-alanine, respectively. That is, chiral Boc-L-alanine alanine amide monomer 1 and non-chiral acrylic acid propionamide monomer 2.The two monomers were homopolymerized under the action of catalyst nbdfen [畏 _ 6-C _ 6B-H _ 5B _ 6H _ 5H _ 3] to obtain three kinds of polymers with average molecular weight of Mn=1.8 脳 104-2.0 x 104. The optical activity of the homopolymer and copolymers of chiral monomers were determined by dichroism, UV-vis-vis and [伪] D. And the polymer chain has a single chiral helical conformation. The effects of side group and non-chiral monomer on the secondary structure of the polymer. The copolymer removed the tert-butoxycarbonyl Boco group from the side chain in the presence of trifluoroacetic acid / dichloromethane to obtain a helical polymer with amino groups. This kind of helical polymer with special functional groups will be used in the next step of grafting reaction. In this thesis, the chiral fluorescent composite microspheres were prepared by using inorganic silica nanoparticles as the carrier. First, 3-aminopropyl trimethoxysilane and. Chemical modification of silica nanoparticles by succinic anhydride, The content of carboxyl group on the surface of silica nanoparticles was studied by titration. Secondly, under the action of dehydrating agent and catalyst, the content of carboxyl group on the surface of silica nanoparticles was studied. The helical polymers with amino groups were grafted onto the surface of silica nanoparticles by covalent bonding. The grafting rate of helical polymers on silica nanoparticles was studied by thermogravimetric curves. The polymer with fluorescent properties is coated on the surface of chiral composite microspheres by the method of deposition polymerization. A novel chiral fluorescent composite microsphere was prepared. In this paper, the surface morphology and particle size of chiral fluorescent composite microspheres were observed by scanning electron microscopy and transmission electron microscope. The spectra show that the chiral fluorescent composite microspheres have some fluorescence properties and this kind of chiral fluorescent composite microspheres will play an important role in the field of biomarker and tracer.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ127.2;TB33

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