倍半硅氧烷—共轭寡聚电解质荧光探针设计合成及生物应用研究

发布时间：2018-03-23 07:23

本文选题：倍半硅氧烷　切入点：共轭寡聚电解质　出处：《南京邮电大学》2015年硕士论文　论文类型：学位论文

【摘要】：多面体低聚倍半硅氧烷(Polyhedral Oligomeric Silsesquioxane,POSS)作为连接有机材料和无机材料的重要中间体材料,受到越来越多的关注。本论文设计合成了POSS与寡聚苯撑乙烯衍生物(OPVE)杂化共轭水溶性荧光纳米颗粒。通过核磁共振谱、飞行时间质谱、热重分析、傅里叶变换-红外光谱仪、紫外分光光度计、荧光分光光度计、荧光寿命谱仪、透射电子显微镜、动态光散射粒径-电位测量仪对纳米粒子的分子结构,物理性能,热性能,光学性能,电致发光性能的表征,反映出POSS-OPVE良好的水溶性、光稳定性、热稳定性、生物相容性、抗氧化性、强度以及低毒性等。本论文对于POSS-OPVE纳米粒子的在纳米生物领域的应用,主要包括以下几个方面内容:1,POSS-OPVE应用于大肠杆菌微生物燃料电池,作为电子运输的媒介体,可以使MFC产生的电压增加,功率提高。并且通过与同类已知共轭寡聚电解质的对照试验我们得知了POSS-OPVE在产电方面比其更有效。显示了POSS-OPVE等有机-无机杂化材料在作为微生物燃料电池媒介体方面提高产电效能方面具有很大潜能。2,POSS纳米颗粒应用于脂多糖(LPS)的检测,由于两者之间的静电吸引相互作用以及有机憎水部分相互排斥作用,观察到POSS-OPVE荧光增强且蓝移,成功地将POSS-OPVE用于革兰氏阴性细菌细胞膜的特异性识别物LPS以及的检测,最高灵敏度可达23 n M。并把其应用革兰氏阴性菌的染色鉴别。
[Abstract]:Polyhedral Oligomeric Silsesquioxaneus POSS (polyhedron oligosilane) is an important intermediate material for connecting organic and inorganic materials. In this paper, the conjugated conjugated water-soluble fluorescent nanoparticles of POSS and OPVE were designed and synthesized by NMR, time-of-flight mass spectrometry (TMS), thermogravimetric analysis (TGA), Fourier transform infrared spectrometer (FTIR), Fourier transform infrared spectroscopy (FTIR), Fourier transform infrared spectroscopy (FTIR). UV Spectrophotometer, fluorescence Spectrophotometer, fluorescence Lifetime Spectrometer, Transmission Electron microscope, dynamic Light scattering Particle size potentiometer, Molecular structure, physical Properties, Thermal Properties, Optical Properties of nanoparticles, The characterization of electroluminescent properties shows that POSS-OPVE has good water-solubility, photostability, thermal stability, biocompatibility, oxidation resistance, strength and low toxicity. The main contents include the following aspects: 1 / 1 POSS-OPVE applied to E. coli microbial fuel cells, as an electronic transport medium, can increase the voltage generated by MFC. Power increase. And through the control test with the same kind of known conjugated oligoelectrolytes, we know that POSS-OPVE is more effective than it in producing electricity. It shows that POSS-OPVE and other organic-inorganic hybrid materials are used as microbial fuel cell vectors. In terms of improving the efficiency of electricity production, it has great potential to be used in the detection of lipopolysaccharide (LPSs) by using poss nanoparticles. Due to the electrostatic attraction interaction and the repulsion of organic hydrophobicity, POSS-OPVE fluorescence was enhanced and blue shift was observed. POSS-OPVE was successfully used in the detection of LPS, a specific cell membrane recognizer of Gram-negative bacteria. The highest sensitivity was up to 23 n M. the method was applied to the identification of Gram-negative bacteria.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TM911.45

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