瓜果类生物质光合细菌产氢试验研究

发布时间：2018-03-31 19:00

  本文选题：瓜果类生物质　切入点：光合细菌　出处：《河南农业大学》2015年硕士论文

【摘要】：中国瓜果生产量遥居世界首位,其中具有代表性的苹果和西瓜年产量分别达到2千万吨和7千万吨。巨大数量的水果产量势必会因为恶劣天气、长途运输、不易保存等原因造成大量的腐烂,科学研究表明人畜食用腐烂水果后会造成中毒甚至死亡,使用腐烂瓜果类生物质进行光合细菌产氢不仅可以消除资源浪费缓解环境污染,还可以产生清洁的可再生能源,因此成为了可再生能源领域的热点研究方向。本文研究了不同p H值对HAU-M1光合细菌生长的影响,做出了其生物量干重对OD660nm值的标准曲线,利用Logistic模型进行了光合细菌生长回归方程模拟。以苹果泥、西瓜皮为试验材料进行光合细菌产氢试验,研究料液比、p H值调控、初始p H值、光照度、发酵温度、接种量等发酵工艺对苹果泥光合生物制氢工艺的影响规律,使用响应面法对苹果泥光合细菌产氢试验进行优化,提出最佳的苹果泥光合生物制氢工艺参数。并对瓜果类生物质与秸秆类生物质光合细菌产氢进行了对比分析。试验结果表明:1)p H值对光合细菌生长及产氢过程都有显著影响,随着碱性增强,光合细菌生物量逐渐增大,当p H值为8时,光合细菌生物量达到最大,为543.714 mg/L,最佳接种时间为84-96h。2)最佳的苹果泥光合细菌产氢单因素条件为:苹果泥与蒸馏水质量比为1:4,初始p H值为7,光照度为3000 lx,温度为30℃,光合细菌接种量为40%。根据响应面法模型预测苹果泥光合细菌产氢量最大值为714.28m L,此时试验工艺条件为:初始p H值7.14,光照度3031.33 lx,温度30.47℃,料液比0.24;最大比产氢量为16.59 m L·g-1,工艺条件为:初始p H值7.14,光照度3032.12 lx,温度30.50℃,料液比0.21。可为瓜果类生物质光合生物制氢工艺技术和理论的进一步研究提供参考。3)纤维素酶对西瓜皮有很好的酶解作用,经纤维素酶处理后,西瓜皮汁液还原糖浓度有显著提高。p H调控不利于光合细菌产氢,p H值调控可能会减弱光合细菌活性或者直接将其杀死,对产氢速率和累积产氢量都有很大抑制作用。4)采用响应面法BBD模型优化玉米秸秆酶解糖化光合细菌产氢试验,获得的最佳产氢条件为:p H为5.43,温度为30.8℃,纤维素酶量为70 mg/g。玉米秸秆为5g时模型最大产氢量为149.39 m L,最大产氢率为29.88m L/g。
[Abstract]:China ranks first in production of melon and fruit in the world, with the representative annual production of apples and watermelons reaching 20 million tons and 70 million tons respectively. A huge amount of fruit production is bound to be transported over long distances because of bad weather. Scientific research shows that human and animal consumption of rotten fruit can lead to poisoning and even death. Using decomposed fruits biomass to produce hydrogen by photosynthetic bacteria can not only eliminate the waste of resources and alleviate environmental pollution. It can also produce clean renewable energy, so it has become a hot research direction in the field of renewable energy. In this paper, the effects of different pH values on the growth of HAU-M1 photosynthetic bacteria were studied, and the standard curve of biomass dry weight to OD660nm value was obtained. The Logistic model was used to simulate the growth of photosynthetic bacteria. The hydrogen production of photosynthetic bacteria was studied by using apple mud and watermelon peel as experimental materials. The control of pH value, initial pH value, light intensity and fermentation temperature were studied. The effects of inoculation and other fermentation techniques on the hydrogen production of apple puree photosynthetic organisms were studied. The response surface method was used to optimize the hydrogen production test of apple puree photosynthetic bacteria. The optimum technological parameters of photosynthetic biological hydrogen production from apple mud were put forward. The hydrogen production of photosynthetic bacteria from melon and fruit biomass was compared with that of straw biomass. The results showed that the value of 1: 1p H had a significant effect on the growth and hydrogen production process of photosynthetic bacteria. With the increase of alkalinity, the biomass of photosynthetic bacteria gradually increased. When pH value was 8, the biomass of photosynthetic bacteria reached the maximum. The optimum conditions for hydrogen production of photosynthetic bacteria from apple mud were as follows: the mass ratio of apple puree to distilled water was 1: 4, initial pH value was 7, light intensity was 3000 LX, temperature was 30 鈩，

本文编号：1692027