高通量微型生物反应器关键技术研究

发布时间：2018-04-11 12:10

本文选题：微型生物反应器 + PLC　；参考：《北京化工大学》2015年硕士论文

【摘要】：随着生物技术的迅猛发展,微生物反应控制系统逐渐由简单的人工操作向自动化、智能化发展。微型生物反应器逐渐成为了生物反应器未来的发展趋势,本文针对生物反应器微型化的问题完成了以下三部分工作：基于PLC研制了一套微型生物反应系统,实现微生物发酵过程中温度、酸碱度(pH)、溶解氧(DO)三个参数的监控,提高了微生物发酵产物产量。首先研究上述三个参数对微生物发酵过程的影响,在此基础上提出各个参数的控制方法,设计了微型生物反应器的硬件架构,同时完成微反应器上、下位机程序的编制。其中上位机程序由昆仑通态组态软件MCGS编制,下位机控制程序由STEP7编制,上、下位机采用RS485通讯。研制了一种基于荧光猝灭原理的光纤氧传感器,用于微生物发酵过程中溶解氧浓度检测。分析了荧光猝灭机理,确定所使用的荧光指示剂。采用聚合物包埋的方法制备了氧传感探头,并对其进行性能表征。根据氧传感探头的荧光特性,设计了荧光强度检测电路。最后以酵母发酵、大肠杆菌发酵为实验对象,初步验证了该传感器的可行性。基于LabVIEW研发了一套的水活度智能控制系统,实现非水相脂肪酶催化过程水活度智能监控。在制定了水活度智能控制系统硬件架构的基础上,结合各配件工作原理运用LabVIEW设计了该控制系统上位机软件程序,该程序具有参数设定、水活度检测、模糊自适应PID控制以及动态数据显示等功能。最后以脂肪酶酯化合成三羟甲基丙烷酯过程中的水活度控制为例,验证了水活度控制系统的控制精度及稳定性等性能。
[Abstract]:With the rapid development of biotechnology, microbial reaction control system has been developed from simple manual operation to automation and intelligence.Micro bioreactor has gradually become the development trend of bioreactor in the future. In this paper, the following three parts have been done to solve the problem of miniaturization of bioreactor: a set of micro bioreactor system based on PLC has been developed.Firstly, the effect of the above three parameters on the fermentation process of microorganism was studied. Based on this, the control method of each parameter was put forward, and the hardware structure of the micro bioreactor was designed. At the same time, the programming of the lower computer on the micro reactor was completed.The upper computer program is compiled by Kunlun on-state configuration software MCGS, and the lower computer control program is compiled by STEP7. The upper and lower computer adopts RS485 communication.An optical fiber oxygen sensor based on fluorescence quenching principle was developed to detect dissolved oxygen concentration during microbial fermentation.The fluorescence quenching mechanism was analyzed and the fluorescence indicator was determined.The oxygen sensor was prepared by polymer embedding and its properties were characterized.According to the fluorescence characteristics of oxygen sensor, a fluorescence intensity detecting circuit is designed.Finally, yeast fermentation and Escherichia coli fermentation were used as experimental objects, and the feasibility of the sensor was preliminarily verified.An intelligent control system of water activity based on LabVIEW is developed to realize intelligent monitoring of water activity in non-aqueous lipase catalytic process.On the basis of establishing the hardware structure of the intelligent control system of water activity, the software program of the upper computer of the control system is designed by using LabVIEW in combination with the working principle of various parts. The program has parameter setting and water activity detection.Fuzzy adaptive PID control and dynamic data display.Finally, the control of water activity in the synthesis of trimethylolpropane ester by lipase esterification is taken as an example to verify the control accuracy and stability of the water activity control system.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ052;TP212

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