精氨酸改性壳聚糖纳米纤维亲和膜的制备及吸附性能研究

发布时间：2018-04-23 18:19

本文选题：亲和膜 + 溶液喷射纺丝技术　；参考：《天津工业大学》2017年硕士论文

【摘要】：亲和膜分离是兼具膜分离和亲和色谱技术的新型生物纯化技术,能实现特异性和高纯化率吸附。随着纳米纤维制备技术的发展,纳米纤维亲和膜技术受到了广泛关注。壳聚糖作为天然的吸附剂,表现出良好的吸附性能,本研究采用壳聚糖衍生物作为亲和膜吸附材料,利用溶液喷射纺丝技术制备纳米纤维亲和膜,将为蛋白质的特异性吸附分离研究提供新思路和方法。本论文首先利用1-(3-二甲氨基丙基)-3-乙基碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)作为催化交联剂,制备了精氨酸/壳聚糖一系列不同取代度的精氨酸壳聚糖(CS-Arg),利用FTIR、13CNMR、XRD、TG和EA等对其结构表征,结果表明随着精氨酸的量增加,取代度呈现出先增大后减小的趋势。论文进一步利用制得的CS-Arg与PLA作为混纺溶液,通过溶液喷射纺丝技术制备纳米纤维(CANFs),通过SEM、接触角、过滤效率及阻力、FTIR和XPS等来表征纳米纤维亲和膜,结果表明,随着取代度的增加,纤维直径稍微变大,平均直径在100～200nm,CANFs膜具有亲水性且有高的过滤效率和低的过滤阻力。将其应用于亲和吸附,对CANFs亲和膜的吸附分离牛血清蛋白性能进行研究,结果表明亲和膜的吸附能力良好,最大吸附量达445.19mg/g;探讨了 pH值、初始浓度、吸附时间对BSA的吸附性能的影响,最佳吸附条件为pH=7.14,吸附时间为10h和初始浓度为3.0mg/mL。对CANFs亲和膜吸附BSA的吸附等温线模型、动力学模型和分子扩散模型研究可知,CANFs亲和膜吸附BSA符合Langmuir模型,属于单分子层吸附,且对BSA的亲和作用在中等范围内,为有效吸附,容易洗脱;吸附速率符合准二级动力学模型,不符合分子扩散模型,分子扩散速率控制步骤为化学吸附作用。CANFs亲和膜对BSA的洗脱研究可知,有很高的再生吸附性能,可以重复使用,稳定性好。本论文通过制备纳米纤维亲和膜介质,研究了其吸附性能和吸附模型,这为新型氨基酸配基的开发、亲和色谱分离过程研究提供相关数据和理论指导,从而拓宽了亲和膜技术的应用领域。
[Abstract]:Affinity membrane separation is a new biological purification technology with both membrane separation and affinity chromatography. With the development of nanofiber preparation technology, nanofiber affinity membrane technology has received extensive attention. Chitosan, as a natural adsorbent, has good adsorption properties. In this study, chitosan derivatives were used as affinity membrane adsorbents, and nano-fiber affinity membranes were prepared by solution jet spinning technique. It will provide a new idea and method for the study of protein specific adsorption and separation. In this paper, 1-HYDIMETHYLSANIMIDINE (1-HYDROXYSucinimide) and N-hydroxy-succinimide (NHS) were first used as cross-linking agents. A series of arginine chitosan (CS-Arg) with different degree of substitution were prepared and characterized by FTIR13CNMR-XRDX TG and EA. The results showed that the degree of substitution increased first and then decreased with the increase of arginine content. In this paper, CS-Arg and PLA were further used as blending solution to prepare nanofibers CANFS by solution jet spinning technology. The affinity membranes were characterized by SEM, contact angle, filtration efficiency and resistance, FTIR and XPS. With the increase of the degree of substitution, the fiber diameter increased slightly, and the average diameter of CANFs membrane was 100 ~ 200nm ~ (-1). The membrane had high hydrophilicity, high filtration efficiency and low filtration resistance. The adsorption and separation of bovine serum protein by CANFs affinity membrane were studied. The results showed that the adsorption capacity of affinity membrane was good, the maximum adsorption capacity was 445.19 mg / g, the pH value and initial concentration of bovine serum protein were discussed. The effect of adsorption time on the adsorption performance of BSA was investigated. The optimum adsorption conditions were pH = 7.14, adsorption time 10 h and initial concentration 3.0 mg 路mL ~ (-1). The adsorption isotherm model, kinetic model and molecular diffusion model for the adsorption of BSA by CANFs affinity membrane showed that the adsorption of BSA by CANFs membrane was in accordance with the Langmuir model and belonged to monolayer adsorption, and the affinity to BSA was in a medium range, which was an effective adsorption. The adsorption rate accords with the quasi-second-order kinetic model, and does not accord with the molecular diffusion model. The control step of molecular diffusion rate is chemisorption. The elution of BSA by CANFs affinity membrane shows that it has high regenerative adsorption performance. Can be reused, good stability. In this paper, the adsorption properties and adsorption models of nanofibers were studied by preparing affinity membrane media, which provided relevant data and theoretical guidance for the development of novel amino acid ligands and the separation process of affinity chromatography. Thus, the application field of affinity membrane technology is widened.
【学位授予单位】：天津工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ051.893

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