一株产电菌Klebsiella oxytoca d7的分离及产电机理

发布时间：2018-05-18 19:51

本文选题：MFC + 生物产电　；参考：《中国环境科学》2017年09期

【摘要】：采用厌氧双层平板法从运行稳定的双室型微生物燃料电池(Microbial fuel cell,MFC)阳极生物膜上分离纯化出一株优势产电纯菌Klebsiella oxytoca,并对其产电机理进行了研究.通过对该纯菌的循环伏安扫描分析,表明该菌具有较强的电化学活性,并且在胞外存在电子受体时就会进行胞外产电呼吸.对该菌培养物进行三维荧光扫描和比较,发现该菌主要是利用腐殖质作为胞外电子传递的介体来完成产电呼吸.铁还原性测试的结果表明过量的3价铁没有给菌株带来额外的增长,该菌对3价铁的还原量有限.研究认为,胞外呼吸不是Klebsiella oxytoca在无氧条件下的主要呼吸方式,而是当有外在电子受体存在时细菌进行胞内呼吸的一种辅助方式,即使环境中存在充分的胞外电子受体,也不会带来Klebsiella oxytoca生长量的显著增加,该菌无法从胞外还原中获得足够的生长需要的能量,仍然主要是通过胞内呼吸获得细胞生长所需的能量,有机物氧化所产生的电子只有少部分用于胞外电子受体的还原.
[Abstract]:A dominant electricity-producing bacterium Klebsiella oxytoca was isolated and purified from the anodic biofilm of biologically stable biofilm of biofilm, Klebsiella oxytoca, and its motor mechanism was studied. The results of cyclic voltammetry showed that the pure strain had strong electrochemical activity and the extracellular electrorespiration occurred when there was an electron receptor outside the cell. Three-dimensional fluorescence scanning and comparison showed that humus was mainly used as the mediator of extracellular electron transfer to complete electrorespiration. The results of iron reductivity test showed that excessive trivalent iron did not increase the strain and that the strain had limited reduction of trivalent iron. It is believed that extracellular respiration is not the main breathing mode of Klebsiella oxytoca under anaerobic conditions, but an auxiliary way for bacteria to breathe in the presence of external electron receptors, even if there are sufficient extracellular electron receptors in the environment. Nor does it lead to a significant increase in Klebsiella oxytoca growth. The bacteria can't get enough energy from extracellular reduction to grow. It still gets the energy it needs to grow mainly through intracellular respiration. Only a few of the electrons produced by organic oxidation are used for the reduction of extracellular electron receptors.
【作者单位】：西安建筑科技大学陕西省环境工程重点实验室教育部西北水资源和环境生态重点实验室;
【基金】：重大水专项(2009ZX07212-002)
【分类号】：TM911.45

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