氧化石墨烯对大鼠角膜上皮细胞的致损伤作用

发布时间：2018-05-24 06:42

本文选题：氧化石墨烯 + 细胞坏死　；参考：《眼科新进展》2017年01期

【摘要】：目的探讨氧化石墨烯(oxidation of graphene,GO)对大鼠角膜上皮细胞的毒性作用。方法体外培养Wistar雌鼠角膜上皮细胞分为正常对照组、GO干预组,正常对照组不做任何处理,GO干预组在培养时加入不同浓度(0.005μg·L~(-1)、0.010μg·L~(-1)、0.020μg·L~(-1)、0.050μg·L~(-1)、0.100μg·L~(-1))的GO。MTT法绘制角膜上皮细胞的生长曲线、检测细胞活性;流式细胞术检测细胞的状态分布;HE染色后显微镜下观察亚细胞结构;用DAPI/PI双染以及台盼兰染色的方法进一步验证经GO干预后的细胞状态。结果经GO干预后的角膜上皮细胞通过HE染色发现细胞内有GO的进入与残留,且细胞形态发生不规则改变,伪足部分出现纤维状结构;MTT结果显示随着GO浓度的增加,细胞活性由(81.55±1.31)%降低至(64.08±0.27)%,随着最低有效浓度的GO(0.005μg·L~(-1))干预时间的增加,细胞活性由(82.61±1.36)%降低至(47.92±1.06)%;流式细胞术显示细胞破碎、死亡的比例随着GO浓度的升高,由52.9%上升至92.8%,而凋亡细胞的比例未发生明显变化;DAPI/PI双染及台盼兰染色结果均显示经GO干预后细胞死亡数目的比例急剧升高(均为P0.01)。结论 GO对大鼠角膜上皮细胞具有毒性作用,提示GO的生物安全性存在隐患。
[Abstract]:Objective to investigate the toxic effect of graphene oxide (graphene oxide) on rat corneal epithelial cells. Methods Wistar female corneal epithelial cells cultured in vitro were divided into two groups: the normal control group was treated with go, and the normal control group was treated with 0. 005 渭 g / L (0. 005 渭 g / L ~ (-1) 0. 010 渭 g / L ~ (-1) L ~ (-1). The GO.MTT method was used to draw the growth curve of the corneal epithelial cells and to detect the activity of the cells in the normal control group (0. 050 渭 g / L ~ (-1) ~ (- 1) ~ 0. 100 渭 g 路L ~ (-1). Flow cytometry (FCM) was used to detect the state distribution of cells and to observe the subcellular structure under microscope after HE staining, and to further verify the cell status after go intervention by DAPI/PI double staining and Trypan blue staining. Results in the corneal epithelial cells treated with go, go was detected by HE staining, and the morphology of the cells changed irregularly. The results of MTT showed that the contents of go increased with the increase of go concentration. The cell activity decreased from 81.55 卤1.31% to 64.08 卤0.27%, and the cell activity decreased from 82.61 卤1.36% to 47.92 卤1.06% with the increase of the minimum effective concentration of GO(0.005 渭 g / L ~ (-1). Flow cytometry showed that the cell was broken and the death rate increased with the increase of go concentration. The percentage of apoptotic cells increased from 52.9% to 92.8%, while the percentage of apoptotic cells did not change significantly. The results of DAPI / Pi double staining and Trypan blue staining showed that the proportion of cell death increased sharply (all P0.01) after go intervention. Conclusion go has toxic effect on corneal epithelial cells of rats, indicating that there are hidden dangers in the biological safety of go.
【作者单位】：兰州大学;
【基金】：国家自然科学基金资助(编号:31471953)~~
【分类号】：TQ127.11;R772.2

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