复合菌种固定化厌氧发酵产氢特性的研究

发布时间：2018-05-28 12:55

本文选题：生物制氢 + 混合菌　；参考：《北京化工大学》2015年硕士论文

【摘要】：在当前气候和能源危机的时代,高热值、清洁能源氢气被认为是最理想的化石能源的替代品。厌氧发酵技术操作条件温和,可以以固体废弃物或废水中的有机生物质为底物,在发酵产氢的同时缓解环境污染问题,成为氢能研究领域的热点。但是传统的悬浮发酵系统存在菌体易被洗出、产氢效率低的缺点。针对以上问题,本论文对从活性污泥中筛选出的两株高效产氢菌进行了共固定化的研究。首先对比了纤维材料、活性炭和生物质吸附材料三种吸附载体对复合菌的固定化效果,得到最佳吸附载体为生物质吸附材料,反复10个批次发酵的平均产氢量为1845 mL,平均产氢得率为1.50 mol H2/mol glucose,比游离发酵提高了62.5%。并且固定化提高了菌体对底物的耐受性,淀粉酶的活性比悬浮发酵提高了2-3倍,己糖激酶的活性比悬浮发酵提高了2倍左右。在以上基础上,还考察了固定化复合菌对木糖的利用情况。结果表明,固定化复合菌能快速利用木糖,最大产氢速度为悬浮发酵的4.5倍。反复10个批次发酵的平均底物利用率为96%,平均产氢量为1972 mL,平均产氢得率为1.47 mol H2/mol xylose,为复合菌以纤维素水解液产氢提供了依据。本论文将从活性污泥中筛选出的两株产氢菌进行共固定化,在保持两株菌协同高效产氢的同时,加快了产氢速度,验证了固定化复合菌反复实验的可行性,为固定化连续发酵产氢奠定了基础。
[Abstract]:In the current climate and energy crisis era, high calorific value, clean energy hydrogen is considered as the ideal alternative to fossil energy. Anaerobic fermentation technology has mild operating conditions and can be used as substrate of solid waste or organic biomass in wastewater to reduce environmental pollution while producing hydrogen by fermentation. It has become a hot spot in the field of hydrogen energy research. However, the traditional suspension fermentation system has the disadvantages of easy washing out of bacteria and low hydrogen production efficiency. In order to solve the above problems, the co-immobilization of two high efficient hydrogen producing bacteria from activated sludge was carried out in this paper. Firstly, the immobilization effect of fiber material, activated carbon and biomass adsorption material on composite bacteria was compared, and the best adsorption carrier was biomass adsorption material. The average hydrogen production was 1845 mL and the average hydrogen yield was 1.50 mol H2/mol glucose, which was 62.5% higher than that of free fermentation. The immobilization improved the substrate tolerance, the activity of amylase was 2-3 times higher than that of suspension fermentation, and the activity of hexokinase was about 2 times higher than that of suspension fermentation. On the basis of above, the utilization of xylose by immobilized compound bacteria was also investigated. The results showed that the immobilized compound bacteria could rapidly utilize xylose and the maximum hydrogen production rate was 4.5 times of that of suspension fermentation. The average substrate utilization ratio was 96%, the average hydrogen production was 1972 mL and the average hydrogen production rate was 1.47 mol H2/mol xylose. it provided the basis for the compound bacteria to produce hydrogen by cellulose hydrolysate. In this paper, two strains of hydrogen producing bacteria selected from activated sludge were co-immobilized. While keeping the two strains producing hydrogen synergistically and efficiently, the rate of hydrogen production was accelerated, and the feasibility of repeated experiments of immobilized compound bacteria was verified. It lays a foundation for continuous fermentation hydrogen production by immobilized.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ929;TQ116.2

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